United Kingdom (UK)
Recombinant monoclonal antibody to IL-1A. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 6H3.1A4.
UniProt Accession Number of Target Protein: P01583
Alternative Name(s) of Target: IL1A; IL-1α; IL1F1; Interleukin-1 alpha; IL-1 alpha; Hematopoietin-1; 6H3.1A4.3E11
Immunogen: The original antibody was generated by immunizing mice with recombinant human IL-1 alpha.
Specificity: This antibody binds human Interleukin-1 alpha, which is a cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems. IL-1α is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells.
Application Notes: The original mouse IgG antibody binds human IL-1A with a binding affinity of Kd= 0.35 nM. The binding characterization of this antibody was done using ELISA. In vitro antibody potency was determined by its ability to inhibit IL-8 production by MRC-5 cells. This antibody was able to inhibit IL-8 production by MRC-5 cells with an IC50 of 2.40E−10M. This antibody was used in combination with 6B12.4F6 (anti-IL-1β) antibody for the generation of anti-human IL-1α/β DVD-Ig molecule that had therapeutic potential (PMID: 20068402).
Antibody first published in:
Wu et al. Molecular construction and optimization of anti-human IL-1α/β dual variable domain immunoglobulin (DVD-Ig™) molecules. MAbs. 2009 Jul-Aug; 1(4): 339–347. PMID:20068402
Note on publication:
Describes the generation of anti-human IL-1α/β DVD-Ig™ molecules using several pairs of monoclonal antibodies with therapeutic potential.