United Kingdom (UK)
Recombinant monoclonal antibody to IL-1B. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 1B12.4H4.
UniProt Accession Number of Target Protein: P01584
Alternative Name(s) of Target: IL1B; IL-1β; 1L1F2; Interleukin-1 beta; IL-1 beta; Catabolin
Immunogen: The original antibody was generated by immunizing mice with recombinant human IL-1 beta.
Specificity: This antibody binds human Interleukin-1 beta, which is a potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. It promotes Th17 differentiation of T-cells and synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. It also plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
Application Notes: The original mouse IgG antibody binds human IL-1B with a binding affinity of Kd= 0.26 nM. The binding characterization of this antibody was done using ELISA. In vitro antibody potency was determined by its ability to inhibit IL-8 production by MRC-5 cells. This antibody was able to inhibit IL-8 production by MRC-5 cells with an IC50 of 5.30E−10M. This antibody was used in combination with 18F4.2C8 (anti-IL-1α) antibody for the generation of anti-human IL-1α/β DVD-Ig molecule that had therapeutic potential (PMID: 20068402).
Antibody first published in:
Wu et al. Molecular construction and optimization of anti-human IL-1α/β dual variable domain immunoglobulin (DVD-Ig™) molecules. MAbs. 2009 Jul-Aug; 1(4): 339–347. PMID:20068402
Note on publication:
Describes the generation of anti-human IL-1α/β DVD-Ig™ molecules using several pairs of monoclonal antibodies with therapeutic potential.