Anti-IL-5 [JES1-39D10]

Description:

Recombinant monoclonal antibody to IL-5. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma JES1-39D10.

Clone Name:

JES1-39D10

Published Application(s):

immunocytochemistry, Block, ELISA, FC, IHC

Published Species Reactivity:

Human

Products based on this clone:

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Ab02207-23.0 Anti-IL-5 [JES1-39D10]
Human
Rabbit IgG
Purified
Ships in 4-5 weeks

Ab02207-7.1 Anti-IL-5 [JES1-39D10]
Human
Rat IgG2a
Purified
Ships in 4-5 weeks

Ab02207-7.4 Anti-IL-5 [JES1-39D10]
Human
Rat IgG2a
Fc Silent™
Purified
Ships in 4-5 weeks

Ab02207-10.0 Anti-IL-5 [JES1-39D10]
Human
Human IgG1
Purified
Ships in 4-5 weeks

Ab02207-10.3 Anti-IL-5 [JES1-39D10]
Human
Human IgG1
Fc Silent™
Purified
Ships in 4-5 weeks

UniProt Accession Number of Target Protein: P05113

Alternative Name(s) of Target: IL5; Interleukin-5; B-cell differentiation factor I; Eosinophil differentiation factor; T-cell replacing factor; TRF
Immunogen: The original antibody was generated by immunizing a male lewis rat with semi-purified recombinant mammalian-expressed IL-5.

Specificity: This antibody recognizes and binds human interleukin-5.

Application Notes: This antibody can effectively block IL-5 induced HL-60 histamine content and TF-1 proliferation. 39D10 was also evaluated for its ability to neutralize the ILJ-dependent growth of eosinophilbasophil colonies (i.e., both histamine-positive and histaminenegative Eo-type) in methylcellulose culture. This antibody did not cross-react with mouse IL-5 in indirect ELISA (PMID: 1706953). This antibody was used in immunohistochemical method to study the localisation and phenotype of individual cytokine producing cells in synovial biopsy specimens in rheumatoid arthritis (PMID:7677442). This antibody was also used in immunocytochemical and a flow cytofluorimetric method to detect intracellular IFN-g, IL-4 and IL-5 in T-cell clones, peripheral blood mononuclear cells PBMC and bronchoalveolar lavage fluid BALF cells (PMID: 9134033). This antibody can be used in combination with JES1-5A10 for the detection of cytokines in a capture ELISA (PMID: 16827896). Humanized version of this antibody CMX5-2 and CMX5-5 had lower affinities for IL-5 than 39D10, with Kd values that were 1.4 times the Kd for 39D10 (PMID: 8844835).

Antibody first published in: Denburg et al. Interleukin-5 is a human basophilopoietin: induction of histamine content and basophilic differentiation of HL-60 cells and of peripheral blood basophil-eosinophil progenitors. Blood (1991); 77(7):1462-8. PMID:1706953
Note on publication: Describes the use of this antibody to study the effect of recombinant human interleukin-5 on myeloid colony formation in methylcellulose and on alkaline passaged HL-60 basophilic cell differentiation.