United Kingdom (UK)
Recombinant monoclonal antibody to IL-9. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma RM9A4.
UniProt Accession Number of Target Protein: P15247
Alternative Name(s) of Target: Interleukin-9; IL9; Cytokine P40; T-cell growth factor P40
Immunogen: This antibody was raised by immunising rats with recombinant full-length murine IL-9.
Specificity: This antibody is specific for murine IL-9.
Application Notes: This antibody, when fluorochrome-conjugated, has been used to achieve intracellular IL-9 staining of T cells, analysed using flow cytometry (Chang et al, 2010; Kim et al, 2010; Horka et al, 2012; Gomez-Rodriguez et al, 2016). This antibody has also been used in a sandwich ELISA to measure IL-9 secretion in supernatants collected from restimulated T cells (Jager et al, 2009).
Antibody first published in:
Jager et al. Th1, Th17, and Th9 Effector Cells Induce Experimental Autoimmune Encephalomyelitis with Different Pathological Phenotypes J Immunol. 2009 Dec 1;183(11):7169-77. PMID:19890056
Note on publication:
Describes the use of this antibody in ELISA analysis to measure cytokine secretion.
Flow-cytometry using the Anti-IL-9 antibody RM9A4 (Ab01262). Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of RM9A4 (Ab01262-23.0, pink line) at a dilution of 1:100 for 1h at RT. The blue line shows unstimulated human peripheral blood leukocytes stained with Ab01262-23.0. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.