Anti-IL-21R [ATR-107]

Description:

Recombinant monoclonal antibody to IL-21R. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody ATR-107.

Clone Name:

ATR-107

Published Application(s):

neutralising, therapeutic

Published Species Reactivity:

Human, Cynomolgus Monkey, Mouse

5

Products based on this clone:

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Ab01293-10.0 Anti-IL-21R [ATR-107]
Human, Cynomolgus Monkey, Mouse
Human IgG1
Purified
In Stock

Ab01293-10.3 Anti-IL-21R [ATR-107]
Human, Cynomolgus Monkey, Mouse
Human IgG1
Fc Silent™
Purified
In Stock

Ab01293-23.0 Anti-IL-21R [ATR-107]
Human, Cynomolgus Monkey, Mouse
Rabbit IgG
Purified
In Stock

Ab01293-2.0 Anti-IL-21R [ATR-107]
Human, Cynomolgus Monkey, Mouse
Mouse IgG2a
Purified
Ships in 4-5 weeks

Ab01293-2.3 Anti-IL-21R [ATR-107]
Human, Cynomolgus Monkey, Mouse
Mouse IgG2a
Fc Silent™
Purified
Ships in 4-5 weeks

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UniProt Accession Number of Target Protein: Q9HBE5

Alternative Name(s) of Target: CD360; Ab01; Ab-01; ATR107; IL21R; Interleukin-21 receptor; IL-21 receptor; NILR; Novel interleukin receptor; Q9JHX3
Immunogen: This antibody was isolated through phage display. The scFv parental clone (18A5), which binds human IL-21R, was isolated from the CS human scFv library, using BaF3 cells expressing human IL-21R and biotinylated IL-21R-Fc fusion protein. Four phage display libraries were constructed based on 18A5, and variants with increased affinity for both human and murine IL-21R were identified, including ATR-107.

Specificity: This antibody recognises both human and murine IL-21R extracellular domains, as both were used as targets during alternating rounds of phage display library panning. This antibody also binds to rat and cynomolgus monkey IL-21R, but does not cross-react with human IL-4Rα or IL-2Rβ.

Application Notes: This antibody binds to both human and murine IL-21R; surface plasmon resonance analysis has revealed this antibody to have an affinity to human and cynomolgus monkey IL-21R of 2 nM, and to murine IL-21R of 16 nM (Vugmeyster et al, 2010). This antibody has been shown to neutralise the IL-21-dependent proliferation of both activated human primary B and CD4+ T cells and mouse CD8+ T cells (Vugmeyster et al, 2010). Additionally, this antibody inhibits IL-21-induced phosphorylation of STAT3 in CD4+ T cells in a concentration-dependent manner (Zhu et al, 2013). In the MRL-Faslpr mouse model of lupus, administration of this antibody has been shown to reduce anti-dsDNA antibodies and IgG deposits in the kidney (Vugmeyster et al, 2010). This antibody did not induce cytokine storm-associated gene activation in human PBMCs in vitro, nor when administered to cynomolgus monkeys (Guo et al, 2010). When administered to humans as part of a phase I trial, this antibody was highly immunogenic, with a prolonged pharmacodynamic effect, but was observed to lead to the development of anti-drug antibodies in 75% of subjects (Hua et al, 2013).

Antibody first published in: Vugmeyster et al. In vitro potency, pharmacokinetic profiles and pharmacological activity of optimized anti-IL-21R antibodies in a mouse model of lupus MAbs. 2010 May-Jun; 2(3): 335–346. PMID:20424514
Note on publication: Describes the original generation and characterisation of this antibody.

Binding Curve

If analysis of Daudi cells using Ab01293-23.0. Click on image for more details.

Binding Curve

Immunofluorescence staining of Daudi cells using anti-IL-21R antibody (Ab01293) ATR-107. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells immobilized on Shi-fix™ cover-slips and stained with the chimeric rabbit IgG version of ATR-107 (Ab01293-23.0) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01293-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (Ab00102-23.0) followed by Alexa Fluor® 488 secondary antibody.

Binding Curve

Flow cytometry on human peripheral blood leukocytes using Ab01293-23.0. Click on image for more details.

Binding Curve

Flow-cytometry using the Anti-IL-21R antibody ATR-107 (Ab01293). Human peripheral blood leukocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, shaded line) or the rabbit IgG version of ATR-107 (Ab01293-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.