United Kingdom (UK)
Recombinant monoclonal antibody to Influenza A nucleoprotein. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from phage display aNP-VHH1.
Alternative Name(s) of Target: αNP-VHH1
Immunogen: The VHH was generated by obtaining a VHH phage library from alpacas immunised with influenza virus PR8. The phage library was subject to three rounds of display-based panning.
Specificity: The antigenic target of αNP-VHH1 is the Influenza A NP. IP confirmed αNP-VHH1 does not show reactivity towards the M1, PB1, PB2 or PA proteins of Influenza A.
Application Notes: Immunoprecipitation confirmed NP as the antigen target of αNP-VHH1 (Ashour et al, 2015). Flow cytometry was used to monitor the inteference of influenza A vRNP nuclear import by αNP-VHH1 (Hanke et al, 2016). In conjunction with negative-stain EM, it was used to propose a model for inhibition of vRNP nuclear import by αNP-VHH1, in which αNP-VHH1 masks the nuclear localisation sequence of NPs (Hanke et al, 2016). Mini-genome assays have shown αNP-VHH1 also interferes with Influenza A replication, in addition to preventing nuclear import of RNPs (Ashour et al, 2015). The complex of αNP-VHH1 and influenza A virus nucleoprotein has been obtained through x-ray crystallography, showing αNP-VHH1 binds to the NP body domain (Hanke et al, 2016).
Antibody first published in:
Ashour et al, 2015. Intracellular Expression of Camelid Single-Domain Antibodies Specific for Influenza Virus Nucleoprotein Uncovers Distinct Features of Its Nuclear Localization J Virol. 2015 Mar;89(5):2792-800 PMID:25540369
Note on publication:
Describes the generation of αNP-VHH1 from a phage display library.