United Kingdom (UK)
Recombinant monoclonal antibody to Integrin alpha V. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 17E6.
UniProt Accession Number of Target Protein: P06756
Alternative Name(s) of Target: 272-17E6; Vitronectin receptor; Vitronectin receptor subunit alpha; CD antigen: CD51; Integrin alpha-V heavy chain; Integrin alpha-V light chain;
Immunogen: The original antibody was generated by immunizing BALB/c mice with of purified placental αvβ3.
Specificity: The antibody reacts with extracellular domains on human αv-integrin chains.
Application Notes: αv-Integrin is an integral membrane protein involved in cell adhesion. Antibody screening by ELISA revealed the antibody reacted specifically with αvβ3. In ELISA on fixed cells (‘CELISA’) the antibody showed a reaction pattern consistent with its recognition of the αv integrin chain (cells expressing αvβ3 and αvβ5 (M21)). The ability of the antibody to recognize native αv integrins was tested by FACS analysis, where the αv-expressing cells (M21) reacted strongly with the antibody. Immunoprecipitation from surface labelled cells showed the antibody detected αv integrin in M21 cells. Furthermore, αv-integrins can function as receptors for vitronectin. However, the antibody had the ability to disrupt stable interaction between vitronectin and αvβ3, and blocked the growth of M21 tumors in nude mice.
Antibody first published in:
Mitjans et al. An anti-alpha v-integrin antibody that blocks integrin function inhibits the development of a human melanoma in nude mice. J Cell Sci. 1995 Aug;108 ( Pt 8):2825-38. PMID:7593323
Note on publication:
The authors describe the generation of an antibody against purified human αvβ3 integrin