United Kingdom (UK)
Recombinant monoclonal antibody to Lewis Y. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 3S193.
Alternative Name(s) of Target: Ley
Immunogen: 3S193 (IgG3) was generated in BALB/c mice by immunisation with Ley-expressing cells of the MCF-7 breast carcinoma cell-line.
Specificity: Lewis Y (Ley) is a blood group antigen expressed at high levels on the surface of epithelial tumors, including small cell lung cancer.
Application Notes: The murine 3S193 showed high specificity for Ley in ELISA tests with synthetic Ley and Ley-containing glycoproteins and glycolipids and also reacted strongly in rosetting assays and cytotoxic tests with Ley-expressing cells (Scott et al, 2000). Murine 3S193 was humanised and in antibody-dependent cell-mediated cytotoxicity assays displayed 100-fold greater activity (Kitamura et al, 1994). 3S193 has been used to screen tumour samples for Ley expression using immunohistochemistry (Krug et al, 2007). A crystal structure of the humanised antibody has been obtained to 1.9 Å resolution (Ramsland et al, 2004).
Antibody first published in:
Kitamura et al, 1994. Specificity analysis of blood group Lewis-y (Le(y)) antibodies generatedagainst synthetic and natural Le(y) determinants. Proc Natl Acad Sci U S A. 1994 Dec 20; 91(26): 12957–12961 PMID:7809154
Note on publication:
Describes the original generation of the antibody and its application to ELISA.