United Kingdom (UK)
Recombinant monoclonal antibody to loggerhead IgY light chain. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma LH9.
Alternative Name(s) of Target: immunoglobulin Y; LC, VL
Immunogen: This antibody was raised by immunizing Balb/c mice with sea turtle IgY pirified using immunoglobulin-binding protein-G.
Specificity: Recognizes all sea turtle IgY light chains, and probably all turtles.
Application Notes: To determine the reactivity of this antibody it was immunoblotted with purified IgY (Rodgers and Rice, 2018; pmid:29787778). This antibody was used as a part of an ELISA created for quantifying bacteria-specific IgY titers in individual turtles (Rodgers and Rice, 2018; pmid:29787778).
Antibody first published in:
Rodgers and Rice Monoclonal antibodies against loggerhead sea turtle, Caretta caretta, IgY isoforms reveal differential contributions to antibody titers and relatedness among other sea turtles Dev Comp Immunol. 2018 Oct;87:12-15. PMID:29787778
Note on publication:
mAb LH12 was developed against the 66 kDa heavy chain of IgY, mAb LH1 was developed against the truncated heavy chain of approximately 37 kDA, and mAb LH9 was developed against the 23 kDa light chains.