United Kingdom (UK)
Recombinant monoclonal antibody to Ly6G. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 1A8.
UniProt Accession Number of Target Protein: P35461
Alternative Name(s) of Target: LGR-1 ; y-6G; GR1; Lymphocyte antigen 6G
Immunogen: LEW/N rats immunized with 1.5x10E8 Ly-6G-transfected EL4J cells
Specificity: Recognises the murine GPI-anchored protein Ly6G, a marker of granulocyte differentiation, maturation and, together with Ly6C, a component of the Granulocyte Receptor-1 Antigen (Gr-1). Unlike another commonly used clone, RB6-8C5, 1A8 does not bind Ly6C.
Application Notes: Neutrophils are crucial components of our immune system. Ly6G is highly expressed on neutrophils, which allows the 1A8 clone to be used not only in detection of neutrophils, but also in selective depletion in vivo. It may also be used as a negative selection marker for bone-marrow derived macrophages, which should be Ly6G(-) and F4/80 (+) (see our F4/80 range of antibodies) . The mouse IgG2a version of the antibody, recombinantly produced by Absolute Antibody, could deplete neutrophils in mice more efficiently than the rat version of the antibody. Further, this version increased depleting effector function, and prevented the production of anti-rat antibodies (Olofsen et al., 2022; PMID: 36359801).
Antibody first published in:
Fleming TJ, Fleming ML, Malek TR. Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. Journal of Immunology PMID:8360469
Note on publication:
Describes production of 1A8 and identification of Ly6G as its target.