United Kingdom (UK)
Recombinant monoclonal antibody to M protein, serotype 5. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 36.2.2.
UniProt Accession Number of Target Protein: P02977
Alternative Name(s) of Target: Streptococcal M-protein; Myosin; Actin; Keratin; Tropomyosin; Troponin; Laminin; Ganglioside; Glycophorin M,N
Immunogen: The original antibody was generated by immunizing BALB/c BYJ mice with streptococcal M type 5 membranes followed by a second, boosting dose of M type 5 streptococcal membranes.
Specificity: This antibody reacts with α-helical molecules. Specifically, it binds streptococcal M-protein (type 5), human myosin, actin, keratin, tropomyosin, troponin, laminin, ganglioside, glycophorin M,N
Application Notes: This antibody reacts with human heart cells and whole streptococci (group A, M type 5), as shown in an ELISA, and human heart cells, human sarcolemma, and group A streptococcal membranes, as shown in a WB (Cunnigham et al., 1984; PMID: 6384047). This antibody reacts with the heavy chain of skeletal muscle myosin. Specifically, it reacts with light meromyosin fragments, as shown in WB and competitive inhibition assays (Cunningham et al., 1986; PMID: 3510006). This antibody labels the cytoplasm of epidermal cells and epidermal appendages in normal and psoriatic skin, as shown by immunofluorescent staining (Swerlick et al., 1986; PMID: 3525693). This antibody binds CV (B3), as shown in an ELISA; neutralizes PV-1 and CV (B3), as shown in a plaque-reduction neutralization assay; and is cytotoxic to rat heart cells and fibroblasts, as shown in a cytotoxicity assay (Cunningham et al., 1992; PMID: 1311095). This antibody reacts with retinal S-antigen (S-Ag), as shown in an ELISA (Lerner et al., 1995; PMID: 8722579). This antibody was found to be cytotoxic for rat heart cells (Antone et al., 1997 ;PMID: 9548482). This antibody binds myosin at a high relative avidity, as determined in a competitive ELISA. (Mertens et al., 2000; PMID: 11282394).
Antibody first published in:
Cunningham et al. Murine monoclonal antibodies reactive with human heart and group A streptococcal membrane antigens Infect Immun. 1984 Oct;46(1):34-41. doi: 10.1128/iai.46.1.34-41.1984 PMID:6384047
Note on publication:
The original publication characterizes the antibody's reactivity with human heart and streptococcal antigens.