United Kingdom (UK)
Recombinant monoclonal antibody to Macaque pan-species IgG. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 1B3.
Alternative Name(s) of Target: Macaque IgG; Macaque immunoglobulin G
Immunogen: This antibody was raised by immunising mice with baboon IgG and screening hybridomas against IgG of different primate species.
Specificity: This antibody reacts with rhesus macaque IgG1 and IgG3, as well a cynomolgus monkey, pigtailed macaque and baboon IgG. It has minimal reactivity with human, mangabey, vervet, chimpanzee, or marmoset IgG.
Application Notes: This anti-macaque antibody has been used in ELISA analysis to detect rhesus anti-mouse antibodies in serum samples (Page et al, 2012). As this antibody is able to distinguish between human and macaque IgG, it has also been used in ELISA analysis to detect macaque antibodies raised against intravenously-administered human HIV broadly neutralising antibodies (Rosenberg et al, 2015). Additionally, it has been used in flow cytometric analysis to confirm the clearance of intravenously-administered anti-CD4 depleting antibody from the plasma of rhesus macaques (Mudd et al, 2011).
Antibody first published in:
Mudd et al. Reduction of CD4+ T cells in vivo does not affect virus load in macaque elite controllers. J Virol. 2011 Jul;85(14):7454-9. PMID:21593153
Note on publication:
This antibody is from the laboratory of Keith Reimann, DVM, Beth Israel Deaconess Medical Center.
ELISA of anti-Macaque pan-species IgG antibody on Cynomolgus monkey IgG1 version of Daclizumab. Binding curves of the HRP-conjugated mouse IgG1 chimeric version of the anti-Macaque pan-species IgG antibody 1B3 (Ab01269-1.1) to an ELISA plate coated with four different versions of Daclizumab: Cynomolgus monkey IgG1 (Ab00187-35.0; blue), human IgG1 (Ab00187-10.0; orange), mouse IgG2a (Ab00187-2.0; grey) and rabbit IgG (Ab00187-23.0; yellow) at a concentration of 2.5 µg/ml. A 3-fold serial dilution from 1000 ng/ml was tested in the experiment. Ab01269-1.1 bound only to the Cynomolgus monkey IgG1 format (Ab00187-35.0).