United Kingdom (UK)
Recombinant monoclonal antibody to Marburg virus nucleoprotein . Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from phage display sdAb 4.
Alternative Name(s) of Target: Marburg virus NP
Immunogen: An unbiased phage display library was generated by extracting RNA from llama blood samples. Phage-display with the MARV-Mus strain was used to select sdAb B from the phage library.
Specificity: sdAb B is specific for MARV-Mus, -Rav, and -Ang strains of the MARV virus but shows no cross-reactivity with representative strains of the 4 EBOV species. The antibody recognises NP, a critical viral structural protein that envelopes the RNA genome.
Application Notes: ELISA was used to establish the specificity of sdAb B for MARV virus strains (Sherwood et al, 2007). sdAb has been crystallised with its antigen NP (Garza et al, 2017). Cross-linking dimeric sdAb to NP restricted viral packaging (Darling et al, 2017).
Antibody first published in:
Goldman et al, 2006. Facile generation of heat-stable antiviral and antitoxin single domain antibodies from a semisynthetic llama library. Anal Chem. 2006 Dec 15;78(24):8245-55. PMID:17165813
Note on publication:
Describes the generation of the phage display library.