Singapore
Recombinant monoclonal antibody to MBP. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from synthetic construct MBP-7128.
UniProt Accession Number of Target Protein: P0AEX9
Alternative Name(s) of Target: malE; MMBP; Maltose-binding periplasmic protein; Maltodextrin-binding protein
Immunogen: The original antibody was generated as a diabody by fusing two different Fv domains by short peptide linkers.
Specificity: This antibody recognizes and binds maltose binding protein of E. coli.
Application Notes: Maltose-binding protein (MBP) is a part of the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. Maltose-binding protein (MBP) is one of the most popular fusion partners being used for producing recombinant proteins in bacterial cells. MBP allows one to use a simple capture affinity step on amylose-agarose columns, resulting in a protein that is often 70-90% pure. In addition to protein-isolation applications, MBP provides a high degree of translation and facilitates the proper folding and solubility of the target protein (PMID: 20978971).
Antibody first published in:
Kim et al. Crystal structures of mono- and bi-specific diabodies and reduction of their structural flexibility by introduction of disulfide bridges at the Fv interface. Sci Rep. (2016); 6; 34515. PMID:27682821
Note on publication:
Describes the crystal structure of the antibody.