Anti-MUC1 [E29]

Description:

Recombinant monoclonal antibody to MUC1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma E29.

Developed in partnership with Ximbio (www.ximbio.com).

Clone Name:

E29

Published Application(s):

WB, IHC

Published Species Reactivity:

Human

6

Products based on this clone:

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Ab01329-2.0 Anti-MUC1 [E29]
Human
Mouse IgG2a
Purified
In Stock

Ab01329-2.3 Anti-MUC1 [E29]
Human
Mouse IgG2a
Fc Silent™
Purified
Ships in 4-5 weeks

Ab01329-23.0 Anti-MUC1 [E29]
Human
Rabbit IgG
Purified
In Stock

Ab01329-10.0 Anti-MUC1 [E29]
Human
Human IgG1
Purified
Ships in 4-5 weeks

Ab01329-10.3 Anti-MUC1 [E29]
Human
Human IgG1
Fc Silent™
Purified
Ships in 4-5 weeks

Ab01329-13.12 Anti-MUC1 [E29]
Human
Human IgG4-S228P
Purified
Ships in 4-5 weeks

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UniProt Accession Number of Target Protein: P15941

Alternative Name(s) of Target: MUC-1; Mucin-1; EMA; Breast carcinoma-associated antigen DF3; Cancer antigen 15-3; CA 15-3; Carcinoma-associated mucin; Episialin; H23AG; Krebs von den Lungen-6; KL-6; PEMT; Peanut-reactive urinary mucin; PUM; Polymorphic epithelial mucin; PEM; Tumor-associated epithelial membrane antigen; Tumor-associated mucin; CD227
Immunogen: This antibody was raised by immunising Balb/c mice with EMA prepared from human milk fat globule.

Specificity: This antibody recognises human EMA which belongs to a familiy of human milk fat globule (HMFG) proteins. It is present in milk as well as in various types of normal and neoplastic epithelia.

Application Notes: E29 antibody has been widely used in studies on various types of neoplastic conditions and is recommended for immunohistochemistry alone or with other antibodies for formalin-fixed, paraffin-embedded tissue characterisation. For instance, Tajima and Koda (2015) used it as a part of immunohistochemical characterisation of the ectomesenchymal chondromyxoid tumor of the tongue. Another group immunohistochemically stained formalin-fixed pancreatic tumour tissue with E29 in order to label EMA (MUC1) (Woei-A-Jin et al., 2016). EMA is frequently positive in meningioma, which can be useful when distinguishing it from other intracranial neoplasms, e.g. Schwannomas. Finally, the absence of EMA staining may also be used as an important factor in tissue characterisation and cancer diagnosis. Lack of EMA signal is typical for certain tumours, such as adrenal carcinoma, seminomas, paraganglioma and hepatoma.

Antibody first published in: Cordell et al. Production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemistry. Br J Cancer. 1985 Sep;52(3):347-54. PMID:3899155
Note on publication: This article describes generaton and characterisation of E29 antibody.

Binding Curve

Western blot on HeLa cell lysate, human breast carcinoma and human colorectal carcinoma. Using Ab01329-23.0. Click on image for more details.

Binding Curve

Western Blot using anti-MUC-1 antibody E29 (Ab01329) HeLa cell lysate (A), human breast carcinoma (B) and human colorectal carcinoma (C) (35µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of E29 (Ab01329-23.0) at 0.1 µg/ml, 0.03 µg/ml and 0.03 µg/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Binding Curve

IF staining of MCF7 cells using Ab01329-23.0. Click on image for more details.

Binding Curve

Immunofluorescence staining of fixed MCF7 cells with anti-MUC-1 antibody E29 (Ab01329) Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of E29 (Ab01329-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01329-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-1.1) followed by staining with Alexa Fluor® 488 secondary antibody.

Binding Curve

Flow cytometry analysis of MCF7 cells using Ab01329-23.0 Click on image for more details.

Binding Curve

Flow cytometry using the Anti-MUC-1 antibody E29 (Ab01329). Paraformaldehyde fixed MCF7 cells were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of E29 (Ab01329-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.