Anti-NAP1L1 [Nb179]

Description:

Recombinant monoclonal antibody to NAP1L1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the alpaca peripheral blood lymphocytes Nb179.

Clone Name:

Nb179

Published Application(s):

WB, ELISA

Published Species Reactivity:

Human

UniProt Accession Number of Target Protein: P55209

Alternative Name(s) of Target: NRP; hNRP; Nucleosome assembly protein 1; NAP-1-related protein
Immunogen: The original antibody was isolated from a VHH library generated by immunization of an alpaca with whole glioblastoma multiforme (GBM) cells.

Specificity: This antibody binds human nucleosome assembly protein 1-like 1 (NAP1L1). This protein is a histone chaperone that plays a role in the nuclear import of H2A-H2B and nucleosome assembly. It also participates in DNA repair mechanisms

Application Notes: This antibody was used for identification of NAP1L1 protein in cytosolic and membrane protein fractions of glioblastoma (GBM) tissues using western blotting. It was reported that NAP1L1 was a good marker to distinguish glioma tissues from reference tissues. ELISA was used for the screening of the nanobody library and identification of specific binders that showed specificity for GBM proteins (PMID: 28498803).

Antibody first published in: Jovčevska et al. Differentially expressed proteins in glioblastoma multiforme identified with a nanobody-based anti-proteome approach and confirmed by OncoFinder as possible tumor-class predictive biomarker candidates. Oncotarget. 2017 Jul 4;8(27):44141-44158. PMID:28498803
Note on publication: Describes the isolation of nanobodies against various antigens overexpressed in glioblastoma multiforme.