United Kingdom (UK)
Recombinant monoclonal antibody to Norovirus capsid protein VP1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the human B cell clone A1227.
UniProt Accession Number of Target Protein: Q83883
Alternative Name(s) of Target: VP1; CP; p59; soluble capsid protein; Protein 30k; p30; Norwalk virus
Immunogen: The original antibody was isolated from an anti-NoV GII.4c IgG serum antibody repertoire generated from three donors that experienced a significant increase in GII.4 titer after immunization with the bivalent GII.4c + GI.1 VLP vaccine.
Specificity: This antibody shows broadly binding but no blocking activity toward multiple GI and GII strains such as GI.I, GII.2, and GII.17. This antibody recognizes a highly conserved epitope located at an occluded site on the norovirus capsid, at the interface between the P domain and shell domain of the Norovirus, which explain its high level of cross-reactivity. Norovirus (NoVs) is one of the main pathogens causing sporadic cases and large outbreaks of acute gastroenteritis, and norovirus can infect people of all ages.
Application Notes: ELISA was used to determine the binding specificity of the antibody for variety of GI and GII genotype VLP. This antibody is capable of recognizing GI.1, GI.3, GI.4, GII.1, GII.2, GII.3, GII.4c, GII.14, GII.17 in an ELISA. This antibody is not capable of neutralizing Norovirus (PMID: 31216462).
Antibody first published in:
Lindesmith et al. Sera Antibody Repertoire Analyses Reveal Mechanisms of Broad and Pandemic Strain Neutralizing Responses after Human Norovirus Vaccination. Immunity. 2019 Jun 18;50(6):1530-1541. PMID:31216462
Note on publication:
Describes the generation of this antibody and study of its crystal structure.
ELISA using A1227 (Ab02975) and Norovirus GII.4 VLP. Binding curves of the rabbit IgG chimeric version of the Anti-Norovirus capsid protein VP1 antibody A1227 (Ab02975-23.0; red line) and isotype control (Ab00102-23.0, anti-fluorescein; yellow line) to an ELISA plate coated with Norovirus GII.4 VLP at a concentration of 2.5 ug/ml. A 3-fold serial dilution from 3000 to 0.0169 ng/ml was performed using Ab02974 antibody. For signal detection, a 1:4000 dilution of a HRP-conjugated anti-rabbit IgG antibody was used.