United Kingdom (UK)
Recombinant monoclonal antibody to Norovirus GII.10. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 5B18.
UniProt Accession Number of Target Protein: Q5F4T5
Alternative Name(s) of Target: Capsid protein
Immunogen: The antibody was generated from a mouse immunized with GII.4 norovirus-445 VLPs.
Specificity: The antibody binds to a face of the GII.10 P1 subdomain close to the S domain and not openly exposed at the VLP surface (residues Val433, Glu496, Asn530, Tyr533, Thr534, and Leu535). The antibody cross-reacts with three GII genotypes GII.4, GII.10, and GII.12 but not with GI genotypes.
Application Notes: The specificity of the IgG format of this antibody was confirmed by ELISA analysis; the antibody detected intact GII.10 VLPs at different pH values with nearly identical efficacy. The structure of the Fab fragment in complex with GII.10 P domain was determined. The Western blotting results showed that the antibody IgG could detect the three GII genotypes (Hansman et al., 2012; PMID: 22278249). The antibody was used for immunofluorescence microscopy to examine VLP binding to fresh biopsies of human ileum and it bound specifically to VLPs (Murakami et al., 2013; PMID: 23799113).
Antibody first published in:
Hansman et al. Structural basis for broad detection of genogroup II noroviruses by a monoclonal antibody that binds to a site occluded in the viral particle. J Virol. 2012 Apr; 86(7): 3635–3646. PMID:22278249
Note on publication:
The paper describes the crystal structure and characterization of the antibody.