United Kingdom (UK)
Recombinant monoclonal antibody to Norovirus P domain. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display Nano-4.
UniProt Accession Number of Target Protein: Q83884
Immunogen: An alpaca was injected with norovirus GII.10 virus-like particles (VLPs), and a phage display library constructed. Nano-4 was selected from this phage display library.
Specificity: Nano-32 is specific for GII.10 strain of Norovirus, but shows limited cross-reactivity with other GI and GII norovirus strains, binding GII.10, GII.17, GII.12, and GII.1 P domains.
Application Notes: The binding specificity of Nano-4 for the P domain of Norovirus strain GII.10 was confirmed using ELISA (Koromyslova et al, 2017). Nano-4 has stronger binding capabilities than Nano-27 or Nano-32; however, it also has limited cross-reactivity for other GI and GII norovirus strains. Nano-4 does not inhibit the binding of GII.10 to A-type saliva, B-type saliva, or pig gastric mucin as shown through neutralisation assays (Koromyslova et al, 2017). The crystal structure of Nano-4 in complex with the GII.17 P domain has been obtained (Koromyslova et al, 2017).
Antibody first published in:
Koromyslova et al, 2017. Nanobodies targeting norovirus capsid reveal functional epitopes and potential mechanisms of neutralization. PLoS Pathog. 2017 Nov 2;13(11):e1006636 PMID:29095961
Note on publication:
Describes the isolation of Nano-4 from a phage display library and its characterisation through ELISA, ITC, and crystallography.