United Kingdom (UK)
Recombinant monoclonal antibody to Notch 1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 2E6.
UniProt Accession Number of Target Protein: P46531
Alternative Name(s) of Target: Notch1; hN1; TAN-1; Neurogenic locus notch homolog protein 1; Translocation-associated notch protein TAN-1
Immunogen: The original antibody was generated by immunizing BALB/c mice with a concatemeric protein containing 4 repeats of amino acids 413-488 of human Notch 1 linked by two non-Notch 1 amino acids (AG linker).
Specificity: This antibody binds human notch 1.
Application Notes: The original human IgG1 version of this antagonist antibody binds notch 1 with an affinity of Kd= 8 nM. This antibody is capable of efficiently blocking the binding of all 4 ligands (Jag1, Jag2, DLL1 and DLL4) to notch 1. This antibody was also capable of specifically inhibiting Notch 1-dependent transcription. In vivo, this antibody was found to show little to no intestinal toxicity in mice. Hu2E6-62 caused decrease in functional angiogenesis by increasing vascular branching and decreasing vascular function. It inhibited angiogenesis induced by the human lung cancer Calu-6 cells. This antibody can be used to inhibit or reduced the proliferation and growth of tumors (WO2012003472).
Antibody first published in: WO2012003472 PMID: