United Kingdom (UK)
Recombinant monoclonal antibody to NS1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody clone n.10.
UniProt Accession Number of Target Protein: Q2F4N6
Alternative Name(s) of Target: Non-structural protein-1; NS1A
Immunogen: The original antibody was originated from a phage display library by panning against the NS1 protein.
Specificity: The antibody is specific for the E domain of the non-structural protein-1. The antibody reacts with viruses belonging to different subtypes and types including avian H5N1, swine H1N1 and H3N2, human H1N1 and H3N2 and influenza B virus. Non-structural protein-1 (NS1) is a multifunctional and conserved protein of influenza viruses. It is produced at the initial phase of the infection for suppressing and evading the host immune responses and enhancing the viral replication.
Application Notes: The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody detected the E domain of NS1 by western blot analysis. The original format of the antibody inhibited influenza virus replication in the infected MDCK cells. The clone could enter the influenza virus infected cells and interfered with the NS1 activities leading to replication inhibition of viruses belonging to different subtypes and types including avian H5N1, swine H1N1 and H3N2, human H1N1 and H3N2 and influenza B virus (Yodsheewan et al. 2013; PMID: 23928258).
Antibody first published in:
Yodsheewan et al. Human monoclonal ScFv specific to NS1 protein inhibits replication of influenza viruses across types and subtypes. Antiviral Res. 2013 Oct;100(1):226-37. PMID:23928258
Note on publication:
The original paper describes the generation and characterization of the antibody.