Anti-paclitaxel [3A3]

Name: Anti-paclitaxel [3A3]
SKU: Ab01157
Availability: InStock

Description:

Recombinant monoclonal antibody to paclitaxel. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 3A3.

Clone Name:

3A3

Published Application(s):

ELISA

Published Species Reactivity:

Products based on this clone:

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Ab01157-1.1 Anti-paclitaxel [3A3]
Mouse IgG1
Purified
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Ab01157-23.0 Anti-paclitaxel [3A3]
Rabbit IgG
Purified
Ships in 4-5 weeks

Alternative Name(s) of Target: PT; PTX; Taxol; 7-Xylosylpaclitaxel; Taxol-7-xyloside; 7-xylosyltaxol
Immunogen: This antibody was raised by immunising BALB/c mice with a solution of 7-xylosyltaxol conjugated to the carrier protein bovine serum albumin (XylTax–BSA).7-xylosyltaxol is a taxol (paclitaxel) derivative, which binds to tubulin and inhibits microtubule dis

Specificity: This antibody is specific for paclitaxel, an anti-neoplastic agent derived from the Pacific yew. It displays low cross-reactivity with other naturally-occurring taxanes.

Application Notes: This antibody has been used in an indirect competitive enzyme-linked immunosorbent assay to determine the paclitaxel content of yew tree samples, with a detection range of 0.098–312.5 μg ml−1 (Chao et al, 2012). An scFv derived from this antibody reacts with both free and immobilised paclitaxel in icELISA analysis, and exhibits less cross-reactivity for paclitaxel-related compounds (Yusakul et al, 2016).

Antibody first published in: Chao et al. Development of an indirect competitive enzyme-linked immunosorbent assay (icELISA) using highly specific monoclonal antibody against paclitaxel J Nat Med (2013) Volume 67, Issue 3, pp 512–518 PMID:23007175
Note on publication: Describes the original generation of this antibody, and its use in an indirect competitive enzyme-linked immunosorbent assay.