Anti-PAI-1 [Nb42 (VHH-2g-42)]

Description:

Recombinant monoclonal antibody to PAI-1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody Nb42 (VHH-2g-42).

Clone Name:

Nb42 (VHH-2g-42)

Published Application(s):

functional assay, inhibit, neutralize

Published Species Reactivity:

Human

UniProt Accession Number of Target Protein: P05121

Alternative Name(s) of Target: SERPINE1; PAI1; PAI; PLANH1; Plasminogen activator inhibitor 1; Endothelial plasminogen activator inhibitor; Serpin E1
Immunogen: The original antibody was isolated from a nanobody library generated by immunization of three alpacas with three different PAI-1 variants.

Specificity: This antibody binds to a conformational epitope on human PAI-1 which comprises the loop connecting strands 1 and 2 of β-sheet B (s1B and s2B) as well as the proximal portions of each strand. Plasminogen activator inhibitor-1 (PAI-1), is a serine protease inhibitor, a unique member of the serpin superfamily which plays an important role in fibrinolysis and is an established risk factor for cardiovascular diseases. It a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading .

Application Notes: This antibody is reported to exhibit moderate dose-dependent PAI-1 inhibition. This antibody is capable of inhibiting PAI-1 activity by directly preventing the initial PAI-1.t-PA complex formation (PMID: 27604413). This antibody interferes with exosite interactions between 37-loop of proteinase and negatively charges residues of the RCL and affects the formation of Michaelis complex and dissociation of acyl enzyme (Komissarov et al, 2019).

Antibody first published in: Zhou et al. Generation and in vitro characterization of inhibitory nanobodies towards plasminogen activator inhibitor 1. Thromb Haemost. 2016 Nov 30;116(6):1032-1040. PMID:27604413
Note on publication: Describes the generation of 5 inhibitory nanobodies against PAI-1.