United Kingdom (UK)
Recombinant monoclonal antibody to PD-1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma J116.
UniProt Accession Number of Target Protein: Q15116
Alternative Name(s) of Target: CD279; PD1; PD 1; Programmed cell death protein 1; Programmed cell death 1; pdcd1; Protein PD-1; hPD-1
Immunogen: The original antibody was raised by immunising BALB/c mice with a human PD-1:IgG1 fusion protein.
Specificity: This antibody is specific for human PD-1.
Application Notes: In immunohistochemical analysis, this antibody has been used to stain for PD-1 expression in human breast cancer frozen tissue sections (Ghebeh et al, 2008), human tonsil cryosections (Iwai et al, 2002), acetone-fixed liver tissue sections (Wang et al, 2011) and formalin-fixed, paraffin-embedded human gastric neuroendocrine carcinoma tissue sections (Yang et al, 2019). This PD-1 blocking antibody has been used, in combination with an anti-PD-L1 antibody, to assess the effects of PD-1 pathway blockade on tumor-infiltrating lymphocytes (Matsuzaki et al, 2010). It has also been used to block PD-1 in samples of human peripheral blood and pleural fluid mononuclear cells (Jurado et al, 2008), and to test the effects of PD-1 blockade on peripheral blood mononuclear cell proliferation and interferon‐γ production in samples from HIV-1-positive patients (Rosignoli et al, 2009).
Antibody first published in:
Iwai et al. Microanatomical localization of PD-1 in human tonsils Immunol Lett. 2002 Oct 1;83(3):215-20 PMID:12095712
Note on publication:
Describes the original generation and characterisation of this antibody.
Western Blot using anti-PD-1 antibody J116 (Ab01418). human tonsil tissue lysate (35µg protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of J116 (Ab01418-1.1) at 0.3 µg/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
Flow cytometry using the Anti-PD-1 antibody J116 (Ab01418). Paraformaldehyde fixed Molt4 cells were stained with anti-unknown specificity antibody (Ab00178-1.1; isotype control, black line) or the mouse IgG1 version of J116 (Ab01418-1.1, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.