United Kingdom (UK)
Recombinant monoclonal antibody to PD-1H. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma mam82.
UniProt Accession Number of Target Protein: Q9D659
Alternative Name(s) of Target: VISTA; VISR; B7-H5; B7H5; GI24; PP2135; SISP1; DD1alpha; C10orf54; chromosome 10 open reading frame 54; V-set immunoregulatory receptor
Immunogen: The antibody was generated by immunising a PD-1H-KO mouse with mouse PD-1H Ig fusion protein and adjuvant.
Specificity: This antibody specifically binds PD-1H.
Application Notes: This antibody has been characterised by ELISA and by staining of PD-1H-transfected P815 mastocytoma. No background staining of WT or PD-1H-KO T cells was observed. This antibody inhibits WT OT-II T cell proliferation at high OVA peptide concentrations (Flies D et al, 2014).
Antibody first published in:
Flies D et al. Coinhibitory receptor PD-1H preferentially suppresses CD4+ T cell–mediated immunity J Clin Invest. 2014 May 1; 124(5): 1966–1975. PMID:24743150
Note on publication:
Describes the generation of the antibody and its use to validate a coinhibitory function of PD-1H.
Immunofluorescence staining of RAW 264.7 cells using anti-PD-1H antibody (Ab01017) mam82. Immunofluorescence analysis of paraformaldehyde fixed RAW 264.7 cells stained with the chimeric rabbit IgG version of mam82 (Ab01017-23.0) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01017-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (Ab00102-23.0) followed by Alexa Fluor® 488 secondary antibody.