United Kingdom (UK)
Recombinant monoclonal antibody to PfMSP-1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 5.2.
UniProt Accession Number of Target Protein: P04933
Alternative Name(s) of Target: PMMSA; P. falciparum merozoite surface protein 1; MSP-1; p195
Immunogen: This antibody was raised by immunising BALB/c mice with schizont-enriched P. falciparum.
Specificity: This antibody specifically recognises a disulphide-dependent conformational epitope of the native Plasmodium falciparum merezoite surface protein 1.
Application Notes: This antibody has been used in indirect immunofluorescence analysis to stain schizonts and free merezoites in acetone-fixed parasite samples (Siddiqui et al, 1986), and in immunoprecipitation (Siddiqui et al, 1986) and ELISA (Yoshida et al, 1999; Mazumdar et al, 2009) experiments. This antibody reacts strongly with native PfMSP-1 in immunoblot and ELISA analysis, but reactivity declines under denatured conditions ( Mazumdar et al, 2009).
Antibody first published in:
Siddiqui et al. Induction of Protective Immunity to Monoclonal-Antibody-Defined Plasmodium falciparum Antigens Requires Strong Adjuvant in Aotus Monkeys Infect Immun. 1986 Apr;52(1):314-8. PMID:3514459
Note on publication:
Describes the original generation of this antibody, and its characterisation in IHC and IP analysis.