Anti-Podoplanin [Pmab-295]

Description:

Recombinant monoclonal antibody to Podoplanin. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma Pmab-295.

Clone Name:

Pmab-295

Published Application(s):

WB, ELISA, FC, IHC

Published Species Reactivity:

Elephant

UniProt Accession Number of Target Protein: G3T5I4

Alternative Name(s) of Target: PDPN; elePDPN; Elephant PDPN; Elephant podoplanin
Immunogen: The original antibody was raised by immunizing BALB/c mice four times with three keyhole limpet hemocyanin-conjugated elePDPN peptides, including ₃₈-EGGMVIPGVEDNMV-₅₁, + C-terminal cysteine, followed by a final booster shot.

Specificity: This antibody is specific for elephant Podoplanin (elePDPN). PDPN is an essential marker of lung type I alveolar cells, kidney podocytes, and lymphatic endothelial cells.

Application Notes: This antibody recognized both elePDPN-overexpressing Chinese hamster ovary (CHO)-K1 cells and endogenous elePDPN-expressing LACF-NaNaI cells by flow cytometry (FC) and Western blotting (WB). Kinetic analyses using FC showed that this antibody's K_D for CHO/elePDPN was 1.5 × 10^-8 M. Furthermore, this antibody detected elePDPN-expressing cells using immunohistochemistry (IHC) (Kudo et al., 2022; PMID: 35917562). An ELISA with the alanine-substituted peptides of the elePDPN extracellular domain (amino acids 38–51) revealed that the binding epitope of this antibody includes Met41, Pro44, and Glu47 of elePDPN (Okada et al., 2022; PMID: 35917553).

Antibody first published in: Kudo et al. Development of a Monoclonal Antibody PMab-295 Against Elephant Podoplanin Monoclon Antib Immunodiagn Immunother. 2022 Aug;41(4):194-201. doi: 10.1089/mab.2022.0007 PMID:35917562
Note on publication: The original publication describes the development and characterization of a novel monoclonal antibody, PMab-295, that specifically recognizes elephant podoplanin (elePDPN).

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