United Kingdom (UK)
Recombinant monoclonal antibody to Podoplanin. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma PMab292.
UniProt Accession Number of Target Protein: G9KFV5
Alternative Name(s) of Target: Pmab-292; PDPN; ferret PDPN; ferPDPN; ferret
Specificity: The antibody is specific for ferret Podoplanin. Podoplanin is a type I transmembrane sialoglycoprotein that plays essential roles in the development of the heart, alveoli, and lymphatic system.
Application Notes: The antibody recognized ferPDPN-overexpressed Chinese hamster ovary-K1 (CHO/ferPDPN) cells by flow cytometry and Western blotting. The kinetic analysis using flow cytometry showed that the KD of the antibody for CHO/ferPDPN was 3.4 × 10-8 M. Furthermore, PMab-292 detected lung type I alveolar epithelial cells, lymphatic endothelial cells, and glomerular/Bowman's capsule in the kidney using immunohistochemistry (Goto et al., 2022; PMID: 35471053).
Antibody first published in:
Goto et al. Development of a Monoclonal Antibody PMab-292 Against Ferret Podoplanin Monoclon Antib Immunodiagn Immunother. 2022 Apr;41(2):101-109. PMID:35471053
Note on publication:
The original paper describes the generation and characterization of the antibody.