Anti-Prostate specific antigen [cAbPSA07]

Description:

Recombinant monoclonal antibody to Prostate specific antigen. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the camel peripheral lymphocytes cAbPSA07.

Clone Name:

cAbPSA07

Published Application(s):

SPR, ELISA

Published Species Reactivity:

Human

UniProt Accession Number of Target Protein: P07288

Alternative Name(s) of Target: PSA; KLK3; APS; Prostate-specific antigen; Gamma-seminoprotein; Seminin; Kallikrein-3; P-30 antigen; Semenogelase; N7; cAbPSA-N7
Immunogen: The original antibody was generated by immunizing a female dromedary (Camelus dromedarius) with purified prostate-specific antigen mized mixed in Gerbu adjuvant. A VHH library was constructed and the antibody was isolated by panning against PSA.

Specificity: This antibody binds human prostate specific antigen (PSA). The antibody also binds to PSA that is associated with ACT.

Application Notes: The specificity of the original format of the antibody was confirmed by ELISA analysis. Enzymatic activity of PSA in the presence or absence of the antibody was tested. The binding affinity of the antibody with three isoforms of PSA (PSAfree, PSA non-ACT-binding, and PSA-ACT complex) were measured by surface plasmon resonance (Kd= 0.16 nM, 0.18 nM and 0.18 nM respectively) (Saerens et al. 2004; PMID: 15459193). The antibody was employed in a surface plasmon resonance biosensor for PSA detection and proved to be a promising method as it enabled the detection of PSA levels as low as 1 ng/mL (Huang et al., 2005; PMID: 16076438). Further, the antibody was employed in a SPR immunosensor for the detection of PSA. The results showed that the biosensor was able to detect PSA at concentrations below 1 ng/ml in 15 min (Saerens et al., 2005; PMID: 16316161).

Antibody first published in: Saerens et al. Single domain antibodies derived from dromedary lymph node and peripheral blood lymphocytes sensing conformational variants of prostate-specific antigen. J Biol Chem. 2004 Dec 10;279(50):51965-72. PMID:15459193
Note on publication: The original paper describes the generation and characterization of the antibody.