United Kingdom (UK)
Recombinant monoclonal antibody to Rabbit IgG. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma A10B.
Alternative Name(s) of Target: immunoglobulin gamma
Immunogen: The antibody was originally generated by immunizing BALB/c mice with purified rabbit IgG.
Specificity: The antibody recognizes the constant heavy chain 1 (CH1) domain of rabbit IgG.
Application Notes: Different formats of the antibody (scFv-cys, scFv, Fab and IgG) were employed for the construction of biosensors used for antigen detection in complex samples. In scFv-cys, one glycine of the linker was replaced with a cysteine, which determine an orientation of the scFv fragments on the sensor surface. The performance, in terms of sensitivity and selectivity, in biosensors prepared with scFv-cys, scFv, Fab and IgG was compared. The scFv-cys based biosensors displayed greater assay sensitivity and exhibited less nonspecific activity than intact monoclonal IgG and Fab fragment-based immunosensors. The scFv-cys biosensor surface was easily regenerated by stripping the rabbit IgG from the surface using an acidic solution. Moreover, western blot analysis showed purified scFv migrated as a single band at a MW of ~32 000 and did not appear to be degraded and the specificity of IgG1, Fab fragment and scFv towards rabbit IgG was determined by ELISA. (PMID: 15679346) In another study, two histidine amino acids were incorporated into the linker peptide of the scFv fragment and the scFv-His fragment was then employed to develop a new biosensor. The specificity of scFv and scFv-Hys were confirmed by ELISA. (PMID: 16255580) The affinity constant of rabbit IgG binding with scFv measured by using a conventional regeneration SPR method ((2.2 ± 1.5) × 10^7 M−1) was comparable with the value determined with a nonregeneration protocol (2.5 ± 0.2) × 10^7 M−1, and quartz crystal microbalance (1.9 × 10^7 M−1). (PMID: 16536419)
Antibody first published in:
Shen et al. Single-chain fragment variable antibody piezoimmunosensors Anal Chem. 2005 February 1; 77(3): 797–805. PMID:15679346
Note on publication:
The paper describes a biosensor with high diagnostic potential for rapid and sensitive detection of antigens in complex biological samples.