United Kingdom (UK)
Recombinant monoclonal antibody to RRV E2. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma D7.
UniProt Accession Number of Target Protein: Q88724
Alternative Name(s) of Target: RRV Envelope gp; Ross River Virus envelope glycoprotein; O’nyong-nyong VLP; Chikungunya VLP; CHIKV-VLP
Immunogen: This antibody was raised by immunizing mice.
Specificity: This antibody is specific for the E2 envelope protein of RRV. This antibody is also reported to cross react with O’nyong-nyong VLP and Chikungunya VLP (CHIKV-VLP).
Application Notes: This antibody binds RRV E2, and has been used to stain RRV-positive cells and in FC analysis of E2 surface expression (Jupille, 2013). This antibody has also been used to detect RRV binding to HeLa cells, demonstrating that synthetic peptides representing the α1β1 integrin binding site on collagen IV are able to inhibit RRV binding to cells, thus implicating the α1β1 integrin as an RRV cell-binding receptor (La Linn, 2005). This antibody can be used for the detection of Ross river virus E2 protein, O’nyong-nyong VLP (ONNV VLP) and Chikungunya VLP (CHIKV-VLP) in an ELISA.
Antibody first published in:
Jupille et al. A Tyrosine-to-Histidine Switch at Position 18 of the Ross River Virus E2 Glycoprotein Is a Determinant of Virus Fitness in Disparate Hosts Journal of Virology, 2013, Vol. 87(10), p.5970 PMID:23514884
Note on publication:
Describes the use of this antibody in FC and labelling studies.