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- Ab00934-1.1 Anti-Shiga toxin 1 [5-5B]
- Escherichia coli
- Mouse IgG1
- Purified
- Ships in 4-5 weeks
- Ab00934-23.0 Anti-Shiga toxin 1 [5-5B]
- Escherichia coli
- Rabbit IgG
- Purified
- In Stock
Recombinant monoclonal antibody to Shiga toxin 1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 5-5B.
UniProt Accession Number of Target Protein: Q8X4M7
Alternative Name(s) of Target: Stx1; stx1B
Immunogen: This antibody was raised by immunising mice with Stx1 toxoid.
Specificity: This antibody is specific for the Stx1 B subunit, and does not recognise Stx2
Application Notes: This antibody has been shown to bind the B subunit of Stx1 in Western blot analysis (Nakao et al, 2002). This antibody is able to neutralise Stx1 cytotoxicity (Nakao, 2002). Flow cytometric experiments have revealed that this antibody binds to free Stx1 B subunits, not cell-bound Stx1, and inhibits Stx1 binding to its receptor on target cells (Nakao et al, 2002). A Fab derived from this antibody also specifically binds Stx1, as determined by ELISA, and neutralises Stx1 cytotoxicity (Inoue et al, 2004).
Antibody first published in:
Nakao et al. Monoclonal Antibody to Shiga Toxin 1, Which Blocks Receptor Binding and Neutralizes Cytotoxicity Microbiol. Immunol., 46(11), 777―780, 2002 PMID:12516775
Note on publication:
Describes the original generation of this antibody, and its use in neutralisation assays and Western blot and FC experiments.