- Ab00324-23.0 Anti-SNCG [SAIC-31A-10]
- Rabbit IgG
- In Stock
- Ab00324-1.1 Anti-SNCG [SAIC-31A-10]
- Mouse IgG1
- In Stock
Recombinant monoclonal antibody to SNCG. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the B-cell cDNA library SAIC-31A-10.
UniProt Accession Number of Target Protein: Q6FHG5
Alternative Name(s) of Target: Synuclein gamma variant; Synuclein, gamma (Breast cancer-specific protein 1) variant
Immunogen: The KLH-conjugated peptide "YLAPSGPSGTLK", derived from Gamma-synuclein. Gamma-synulein plays a role in neurofilament network integrity.
Specificity: Recognises human SNCG.
Application Notes: Original data characterizing this antibody may be found <a href="http://antibodies.cancer.gov/apps/site/detail/synuclein%2C%20gamma%20(breast%20cancer-specific%20protein%201)%20Peptide%201#synuclein,_gamma_(breast_cancer_specific_protein_1)_Peptide_1" tar
Antibody first published in:
Schoenherr et al. Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Mol Cell Proteomics PMID:25512614
Note on publication: Describes generation a panel of recombinant antibodies using synthetic peptides of human proteins as well as validation using ELISA and multiple reaction monitoring-mass spectrometry.
Flow cytometry using the Anti-SNCG antibody SAIC-31A-10 (Ab00324). Paraformaldehyde fixed U251 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of SAIC-31A-10 (Ab00324-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Immunofluorescence staining of fixed U251 cells with anti-SNCG antibody SAIC-31A-10 (Ab00324). Immunofluorescence analysis of paraformaldehyde fixed U251 cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-31A-10 (Ab00324-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00324-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.