United Kingdom (UK)
Recombinant monoclonal antibody to SPLCV Coat protein. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the yeast display library scFvF7.
UniProt Accession Number of Target Protein: E5G5X2
Alternative Name(s) of Target: Sweet potato leaf curl virus; SPLCV capsid protein; SPLCV coat protein; AV1; V1; capsid protein; coat protein SPLCV-F7
Immunogen: The original antibody was isolated from a yeast display library by two rounds of screening against Sweet potato leaf curl virus (SPLCV).
Specificity: This antibody recognizes and binds the Sweet potato leaf curl virus (SPLCV), which causes yield losses in sweet potato cultivation. The sweet potato leaf curl virus, a member of the genus Begomovirus, is transmitted by the whitefly (Bemisia tabaci Genn.), which is the only natural vector. SPLCV causes symptoms including upward leaf curling in young stage and is responsible for declining yields around the world.
Application Notes: The binding characterization of this antibody for sweet potato leaf curl virus (SPLCV) coat protein was done using ELISA. This antibody can also bind SPLCV-infected sweet potato samples of leaves and infected phloem tissue. This antibody was also expressed as a bivalent scFv protein to increase antigen-binding affinity (PMID: 32415170).
Antibody first published in:
Cho et al. Development of novel detection system for sweet potato leaf curl virus using recombinant scFv. Sci Rep. 2020 May 15;10(1):8039. PMID:32415170
Note on publication:
This paper describes the isolation of two scFv antibodies directed against sweet potato leaf curl virus.