Anti-TeA [3F9]

Description:

Recombinant monoclonal antibody to TeA. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody 3F9.

Clone Name:

3F9

Published Application(s):

ELISA

Published Species Reactivity:

Specie independent

Alternative Name(s) of Target: Alternaria mycotoxin tenuazonic acid; Nb-3F9
Immunogen: The original antibody was generated by immunizing a male Bactrian camel with TeA-CMO-KLH followed by antibody library construction and phage display-based selection.

Specificity: The antibody is specific for TeA. The antibody does not cross-react with AOH, AME, DON, ITeA and ZEN. TeA is a tetramic acid derivative, and it is considered to have the highest toxicity and exposure among the Alternaria toxins. The contamination of TeA in foods has widely been a concern for food safety control.

Application Notes: The specificity of the original version of the antibody and the bifunctional fusion protein based on the antibody fused with nanoluciferase (Nb-3F9-Nluc) was confirmed by ELISA analysis (IC50= 89.5 ng/mL and 80.3 ng/mL, respectively). Chemiluminescent enzyme immunoassay (CLEIA) based on Nb-3F9 and bioluminescent enzyme immunoassay (BLEIA) based on Nb-3F9-Nluc were developed for the detection of trace TeA in foodstuff. Under the optimal assay conditions, the IC50 and limit of detection (LOD) value of CLEIA were 8.6 ng/mL and those of BLEIA were 9.3 ng/ml (Wang et al., 2020; PMID: 32702970).

Antibody first published in: Wang et al. Chemiluminescent Enzyme Immunoassay and Bioluminescent Enzyme Immunoassay for Tenuazonic Acid Mycotoxin by Exploitation of Nanobody and Nanobody-Nanoluciferase Fusion Anal Chem. 2020 Sep 1;92(17):11935-11942. doi: 10.1021/acs.analchem.0c02338. Epub 2020 Aug 11. PMID:3270297
Note on publication: The paper describes the generation and characterization of the antibody by phage display selection.