Anti-Tetraspanin 1 [4/12]

Name: Anti-Tetraspanin 1 [4/12]
SKU: Ab01430
Availability: InStock

Description:

Recombinant monoclonal antibody to Tetraspanin 1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 4/12.

German Cancer Reserach Center (DKFZ)

Clone Name:

4/12

Published Application(s):

ELISA, IF, IHC

Published Species Reactivity:

Human

Products based on this clone:

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Ab01430-1.1 Anti-Tetraspanin 1 [4/12]
Human
Mouse IgG1
Purified
In Stock

Ab01430-10.0 Anti-Tetraspanin 1 [4/12]
Human
Human IgG1
Purified
Ships in 4-5 weeks

Ab01430-10.3 Anti-Tetraspanin 1 [4/12]
Human
Human IgG1
Fc Silent™
Purified
Ships in 4-5 weeks

Ab01430-13.12 Anti-Tetraspanin 1 [4/12]
Human
Human IgG4-S228P
Purified
Ships in 4-5 weeks

Ab01430-23.0 Anti-Tetraspanin 1 [4/12]
Human
Rabbit IgG
Purified
In Stock

UniProt Accession Number of Target Protein: O60635

Alternative Name(s) of Target: Net1; C4.8; Tetraspanin-1; Tspan-1; TSPAN1; Tetraspan NET-1; Tetraspanin TM4-C; TM4SF
Immunogen: This antibody was raised by immunising BALB/c mice with the major extracellular domain of tetraspanin 1.

Specificity: This antibody is specific for the largest extracellular domain of tetraspanin 1.

Application Notes: The specificity of this antibody for tetraspanin 1 has been confirmed in ELISA analysis (Hölters et al, 2013). This antibody has been used in immunohistochemical analysis of tetraspanin 1 expression in paraffin-embedded sections of normal and cancerous cervical tissue arrays (Hölters et al, 2013), and in frozen tissue samples. This antibody has also been used for immunocytochemical staining for tetraspanin 1 expression, in SiHa cells following adenoviral transduction (Hölters et al, 2013).

Antibody first published in: Hölters et al. Tetraspanin 1 promotes invasiveness of cervical cancer cells Int J Oncol. 2013 Aug;43(2):503-12. PMID:23754316
Note on publication: Describes the original generation of this antibody, and its use in immunohistochemical analysis.

IF staining of A431 cells using Ab01044-23.0. Click on image for more details.

Immunofluorescence staining of fixed A431 cells with anti-Tetraspanin 1 antibody 4/12 (Ab01430) Immunofluorescence analysis of paraformaldehyde fixed A431 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version 4/12 (Ab01430-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01430-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-1.1) followed by staining with Alexa Fluor® 488 secondary antibody.

Flow cytometry analysis of A431 cells using Ab01430-23.0. Click on image for more details.

Flow cytometry using the Anti-Tetraspanin 1 antibody 4/12 (Ab01430). Paraformaldehyde fixed A431 cells permeabilized with 0.5% triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of 4/12 (Ab01430-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.