United Kingdom (UK)
Recombinant monoclonal antibody to Tissue factor. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 5G9.
UniProt Accession Number of Target Protein: P13726
Alternative Name(s) of Target: TF; platelet tissue factor; factor III; thromboplastin; CD142
Immunogen: Human TF.
Application Notes: The antibody binds specifically to TF, a hemostatic protein which is involved in the extrinsic pathway of coagulation. TF is receptor for circulating serine protease factor VIIa, activating the catalytic VIIa protease domain. The complex formed functions to activate the zymogen factor X into activated factor Xa, which then catalyses the formation of Prothrombin to Thrombin. The pathways results in a fibrin clot which acts to stop bleeding. The monoclonal antibody 5G9 acts as a competitive inhibitor of factor X, thereby inhibiting its activation by the TF.VIIa complex and the formation of a clot.
Antibody first published in:
Ruf W, Edgington TS. An anti-tissue factor monoclonal antibody which inhibits TF.VIIa complex is a potent anticoagulant in plasma. Thromb Haemost. 1991 Nov 1;66(5):529-33. PMID:1803616
Note on publication:
Describes the production of a murine monoclonal antibody directed against TF, and inhibits the complex of TF which Factor VIIa, thereby acting as an anticoagulant in plasma.