United Kingdom (UK)
Recombinant monoclonal antibody to Tissue factor. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma ATR-5.
UniProt Accession Number of Target Protein: P13726
Alternative Name(s) of Target: CD142; TF; hTF; F3; Coagulation factor III; Thromboplastin; platelet tissue factor; factor III
Immunogen: The original antibody was generated by immunizing 5 weeks old male BALB/c mice subcutaneously into the abdomen with purified human tissue factor in equal quantity of Freund's complete adjuvant.
Specificity: This antibody binds human tissue factor (TF) a hemostatic protein which is involved in the extrinsic pathway of coagulation. TF is receptor for circulating serine protease factor VIIa, activating the catalytic VIIa protease domain. The complex formed functions to activate the zymogen factor X into activated factor Xa, which then catalyzes the formation of Prothrombin to Thrombin. The pathways results in a fibrin clot which acts to stop bleeding. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
Application Notes: The binding of this antibody to human tissue factor expressed on the surface of human bladder carcinoma cells J82 was done using whole cell ELISA. This neutralizing activity of this antibody against the FX-binding was measured using the human placenta-derived thromboplastin. This antibody was also capable of inhibiting plasma coagulation (US6677436).
Antibody first published in: PMID: