United Kingdom (UK)
Recombinant monoclonal antibody to TNFalpha. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody 2TY.
UniProt Accession Number of Target Protein: P01375
Alternative Name(s) of Target: TNFSF2; tumor necrosis factor alpha; TNF; C1K3N5; TNFA; Cachectin; TNF-alpha; TNF-a; Tumor necrosis factor ligand superfamily member 2; TNF-α
Immunogen: Affinity selection procedures were used to isolate this scFv from a naïve Tomlinson I phage display library that specifically binds to TNF-α. The panning procedure was carried out by incubating immunotubes coated with recombinant TNF-α protein with a phage suspension. Phage particles with affinity for recombinant TNF-α were amplified in TG-1 bacteria, and three rounds of panning were performed to select high affinity and specificity scFvs.
Specificity: This scFv is specific for human TNF-α.
Application Notes: The binding affinity of this scFv for human TNF-α has been confirmed in ELISA analysis (Safarpour et al, 2018). This scFv could have future therapeutic applications as a TNF inhibitor. TNF inhibition is a strategy currently showing promise in the treatment of a range of inflammatory diseases, including rheumatoid arthritis and Chron's disease.
Antibody first published in:
Safarpour et al. Isolation and Characterization of Novel Phage Displayed scFv Fragment for Human Tumor Necrosis Factor Alpha and Molecular Docking Analysis of Their Interactions Iran J Pharm Res. 2018 Spring; 17(2): 743–752. PMID:29881431
Note on publication:
Describes the original generation of this scFv by phage display.