Anti-TrkB.T1 [FH1D12]

Name: Anti-TrkB.T1 [FH1D12]
SKU: Ab02622
Availability: InStock

Description:

Recombinant monoclonal antibody to TrkB.T1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma FH1D12.

Clone Name:

FH1D12

Published Application(s):

WB, FC, IHC

Published Species Reactivity:

Human, Mouse

Products based on this clone:

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Ab02622-3.0 Anti-TrkB.T1 [FH1D12]
Human, Mouse
Mouse IgG2b
Purified
In Stock

Ab02622-3.3 Anti-TrkB.T1 [FH1D12]
Human, Mouse
Mouse IgG2b
Fc Silent™
Purified
In Stock

Ab02622-10.0 Anti-TrkB.T1 [FH1D12]
Human, Mouse
Human IgG1
Purified
Ships in 4-5 weeks

Ab02622-10.3 Anti-TrkB.T1 [FH1D12]
Human, Mouse
Human IgG1
Fc Silent™
Purified
Ships in 4-5 weeks

Ab02622-23.0 Anti-TrkB.T1 [FH1D12]
Human, Mouse
Rabbit IgG
Purified
In Stock

UniProt Accession Number of Target Protein: Q16620

Alternative Name(s) of Target: BDNF/NT-3 growth factors receptor; NTRK2; GP145-TrkB; Trk-B; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; Tropomyosin-related kinase B
Immunogen: This antibody was raised by immunizing male and female 20-week-old C57BL6 mice that were characterized as genetic null for TrkB.T1 with TrkB.T1 peptide Ac-C(dPEG4)FVLFHKIPLDG-OH maleimide coupled to KLH carrier protein.

Specificity: This antibody is specific for TrkB.T1 peptide Ac-C(dPEG4)FVLFHKIPLDG. TrkB.T1 is a receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity.

Application Notes: To validate this antibody, immunohistochemistry was preformed on multi-tissue block containing human brain, mouse brain, TrkB.T1−/− mouse brain, a cell pellet over-expressing TrkB.T1 and a cell pellet without TrkB.T1 were stained using the scFv version of this antibody. Furthermore, the specificity of this antibody was proven using western blot with the mouse version of this clone. Finally, flow cytometry confirmed the specificity of the mouse version of this antibody (Patwell et al, 2020; pmid:32532995).

Antibody first published in: Patwell et al. A kinase-deficient NTRK2 splice variant predominates in glioma and amplifies several oncogenic signaling pathways Nat Commun. 2020 Jun 12;11(1):2977. PMID:32532995
Note on publication: A role for a truncated NTRK2 splice variant, TrkB.T1, in human glioma was shown.

IF staining of U251 cells using Ab02622-23.0 Click on image for more details.

Immunofluorescence staining of U251 cells with anti-TrkB.T1 (Ab02622) FH1D12 Immunofluorescence analysis of paraformaldehyde fixed U251 cells stained with the chimeric rabbit IgG version of FH1D12 (Ab02622-23.0) (1:1000 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab02622-23.0, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody (Ab00102-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.