Anti-Tu translation elongation factor mitochondrial [Nb225]

Description:

Recombinant monoclonal antibody to Tu translation elongation factor mitochondrial. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the alpaca peripheral blood lymphocytes Nb225.

Clone Name:

Nb225

Published Application(s):

WB, ELISA

Published Species Reactivity:

Human

UniProt Accession Number of Target Protein: P49411

Alternative Name(s) of Target: TUFM; Elongation factor Tu; EF-Tu; P43
Immunogen: The original antibody was isolated from a VHH library generated by immunization of an alpaca with whole glioblastoma multiforme (GBM) cells.

Specificity: This antibody binds human Tu translation elongation factor, mitochondrial (TUFM). This protein promotes GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Also plays a role in the regulation of autophagy and innate immunity. These are chaperone-like properties involved in protein folding and overexpressed in colorectal carcinoma.

Application Notes: This antibody was used for identification of TUFM protein in cytosolic and membrane protein fractions of glioblastoma (GBM) tissues using western blotting. It was reported that TUFM expression was high in GBM compared to reference samples. ELISA was used for the screening of the nanobody library and identification of specific binders that showed specificity for GBM proteins (PMID: 28498803).

Antibody first published in: Jovčevska et al. Differentially expressed proteins in glioblastoma multiforme identified with a nanobody-based anti-proteome approach and confirmed by OncoFinder as possible tumor-class predictive biomarker candidates. Oncotarget. 2017 Jul 4;8(27):44141-44158. PMID:28498803
Note on publication: Describes the isolation of nanobodies against various antigens overexpressed in glioblastoma multiforme.