United Kingdom (UK)
Recombinant monoclonal antibody to Urease alpha subunit. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma HpU-2.
UniProt Accession Number of Target Protein: P14916
Alternative Name(s) of Target: urease-alpha; alpha-urease; urease
Immunogen: HpU-2 was prepared by subcutaneous injection of purified H. pylori urease into BALB/c mice.
Specificity: HpU-2 binds specifically to H. pylori at the epitope with sequence SVELIDIGGNRRIFGFNALVDR - the sequence is part of a loop near to the C-terminus of the alpha subunit of urease (the beta subunit possesses the active site). HpU-18 competes with HpU-2 for binding to this epitope. H. pylori is a gram negative spiral bacterium and causes numerous gastroduodenal diseases. It expresses large amounts of urease when present in the human stomach; urease is key for the survival and pathogenesis of the bacteria as it neutralizes the acidity in the stomach by hydrolysis of urea into ammonia.
Application Notes: HpU-2 results in ~82% inhibition (allosteric inhibition) of urease activity and the inhibitory activity is increased to 87% when HpU-2 is used in combination with HpU-19. HpU-2 has been shown be unsuccessful for use in IHC of formalin-fixed sections of H. pylori-infected human cells. HpU-2 can also be used in SPR and ELISA studies.
Antibody first published in:
Fujii et al. Epitope mapping and features of the epitope for monoclonal antibodies inhibiting enzymatic activity of Helicobacter pylori urease. Biotechnol Bioeng. 2004 May 20;86(4):434-44. PMID:15112296
Note on publication:
Describes the investigation into the inhibitory mechanism of HpU-2 against H. pylori urease. The binding epitope was determined by SPR and molecular modelling.