- Ab00169-1.1 Anti-VAP-1 [2D10]
- Mouse IgG1
- In Stock
- Ab00169-23.0 Anti-VAP-1 [2D10]
- Rabbit IgG
- In Stock
Recombinant monoclonal antibody to VAP-1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 2D10.
UniProt Accession Number of Target Protein: Q16853
Alternative Name(s) of Target: Vascular adhesion protein 1; VAP1; Copper amine oxidase; HPAO; Membrane primary amine oxidase; Semicarbazide-sensitive; AOC3; Amine oxidase, copper containing 3; amine oxidase; amine oxidase; Vascular adhesion protein 1; SSAO; copper-containing amine:oxyg
Immunogen: Affinity purified VAP-1 from tonsil stroma of human origin.
Specificity: Recognises human Vascular Adhesion Protein-1 (VAP-1), a glycosylated homodimeric membrane protein consisting of two 90 kDa subunits connected by disulfide bonds. 2D10 is a conformational specific antibody which recognizes an epitope present only in the dimeric form of VAP-1. Elevated soluble VAP-1 serum- levels have been described in several inflammatory- diseases as well as colorectal cancer.
Antibody first published in:
Kurkijärvi R, Adams DH, Leino R, Möttönen T, Jalkanen S, Salmi M. Circulating form of human vascular adhesion protein-1 (VAP-1): increased serum levels in inflammatory liver diseases. J Immunol. 1998 Aug 1;161(3):1549-57. PMID:9686623
Note on publication: Describes the production of monoclonal antibody against VAP-1 (2D10) and its use in immunoblotting to identify that circulating soluble VAP-1 in serum exists primarily as a dimer.
Immunofluoresence staining of HeLa cells with anti-VAP-1 antibody 2D10. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton and stained with the chimeric rabbit version of 2D10 (Ab00169-23.0) at 10ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00169-23.0, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor® 488 secondary antibody.