Anti-VAP-1 [TK8-14]

Description:

Recombinant monoclonal antibody to VAP-1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma TK8-14.

Clone Name:

TK8-14

Published Application(s):

IP, WB, ELISA, FC, IHC

Published Species Reactivity:

Human

Products based on this clone:

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Ab00168-2.0 Anti-VAP-1 [TK8-14]
Human
Mouse IgG2a
Purified
In Stock

Ab00168-23.0 Anti-VAP-1 [TK8-14]
Human
Rabbit IgG
Purified
Ships in 4-5 weeks

UniProt Accession Number of Target Protein: Q16853

Alternative Name(s) of Target: Vascular adhesion protein 1; VAP1; Copper amine oxidase; HPAO; Membrane primary amine oxidase; Semicarbazide-sensitive; AOC3; Amine oxidase, copper containing 3; amine oxidase; amine oxidase; Vascular adhesion protein 1; SSAO; copper-containing amine:oxy
Immunogen: Affinity purified VAP-1 from tonsil stroma of human origin.

Specificity: Recognises human Vascular Adhesion Protein-1 (VAP-1), a glycosylated homodimeric membrane protein consisting of two 90 kDa subunits connected by disulfide bonds. Epitope recognized by TK8–18 is present both in dimeric and monomeric forms of VAP-1. Elevated soluble VAP-1 serum- levels have been described in several inflammatory- diseases as well as colorectal cancer. TK8-14 is a function-blocking mAb.

Antibody first published in: Kurkijärvi R, Adams DH, Leino R, Möttönen T, Jalkanen S, Salmi M. Circulating form of human vascular adhesion protein-1 (VAP-1): increased serum levels in inflammatory liver diseases. J Immunol. 1998 Aug 1;161(3):1549-57. PMID:9686623
Note on publication: Describes the production of monoclonal antibody against VAP-1 (TK8-14) and its use as a detection antibody, in studying serum level of soluble VAP-1, in a sandwich ELISA.

IF staining of HeLa cells using Ab00168. Click on image for more details.

Immunofluoresence staining of HeLa cells with anti-VAP-1 antibody TK8-14. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton and stained with the chimeric rabbit version of TK8-14 (Ab00168-23.0) at 10ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00168-23.0, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor® 488 secondary antibody