Anti-Vimentin [VB6]

Description:

Recombinant monoclonal antibody to Vimentin. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the alpaca peripheral blood lymphocytes VB6.

Clone Name:

VB6

Published Application(s):

WB, ELISA, IF

Published Species Reactivity:

Human

UniProt Accession Number of Target Protein: P08670

Alternative Name(s) of Target: VIM
Immunogen: The original antibody was generated by immunizing an alpaca with recombinant human vimentin.

Specificity: This antibody binds the human vimentin. Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Application Notes: This antibody can be used for identification of human vimentin using ELISA. A chromobody (nanobody fused with fluorescent protein) generated using this antibody was used for internal tracing of vimentin in various cellular assays. ATTO488 labelled version of this antibody can be used for the immunofluorescent detection of vimentin in MDCK cells. The ATTO488 labelled bivalent dimer version of this antibody (VB6-VB6ATTO488) was also usefully in detection of vimentin in western blot. This antibody was reported to bind human vimentin with a binding affinity of 37 nM (PMID: 26292717).

Antibody first published in: Maier et al. Real-time analysis of epithelial-mesenchymal transition using fluorescent single-domain antibodies. Sci Rep. 2015 Aug 21;5:13402. PMID:26292717
Note on publication: Describes the generation of anti-vimentin chromobodies for tracing vimentin in various cellular assays.