Tumor Suppressor Antibodies

Tumor suppressor proteins are crucial for maintaining cell balance and curbing uncontrolled growth and studying them and their possible loss-of-function is key in advancing cancer research. When tumor suppressors are inactive, cancer can proliferate. Antibodies serve as vital tools in understanding tumor suppressor proteins, allowing precise detection and localization in experiments. Absolute Antibody is committed to supporting scientific progress by offering antibodies tailored for tumor suppressor research. By leveraging these antibodies, researchers gain a refined toolkit to explore and manipulate tumor suppressor functions, paving the way for innovative cancer therapies and contributing to the broader landscape of oncological research.

Key Tumor Suppressor Proteins

P53

The p53 tumor suppressor protein regulates cell growth and prevents the formation of tumors. It acts as a “guardian of the genome” (Park et al., 2016), overseeing DNA repair and, when necessary, triggering apoptosis to eliminate damaged cells. P53 antibody studies are vital because they allow researchers to investigate the protein’s levels, activity, and interactions with precision. Understanding p53’s role and dysregulation in cancer is essential for developing targeted therapies and improving overall strategies for cancer treatment.

Anti-p53 [PAb421]

 

Immunofluorescence staining of fixed A431 with anti-p53 antibody PAb421 (Ab00142). Published applications of this clone are IHC-P, in vivo, IP, WB, ELISA, IF, and IHC-Fr.

Anti-p53 (a.a. 16-25) [X77]

 

Immunofluorescence staining of A431 cells with anti-p53 (a.a. 16-25) (Ab02776) X77. Published applications of this clone are IP, WB, ELISA, and IF.

Anti-p63 [BU5]

 

Immunofluorescence staining of A431 cells with anti-p63 (Ab00871) BU5. Published applications of this clone are FC and IF.

TP63 

TP63, a member of the p53 tumor suppressor gene family, encodes for the p63 protein, which regulates epithelial development and maintains genomic stability (Melino, 2011). Unlike p53, TP63 is not commonly associated with direct tumor suppression but is pivotal for controlling cellular differentiation and maintaining epithelial integrity. Multiple promoters and splice variants give rise to various isoforms of this protein. Studying TP63 in cancer research using antibodies is important for understanding its expression patterns, isoform-specific functions, and potential involvement in various cancers. TP63 antibody assays give researchers insight into the roles of TP63 in normal and aberrant cellular processes and thus its potential implications in cancer development and progression. TP63’s distinct functions in epithelial biology differentiate it from traditional tumor suppressors, making it a subject of interest for researchers investigating its nuanced contributions to cancer biology.

P16 

The p16 tumor suppressor protein, encoded by the cyclin-dependent kinase inhibitor 2A (CDKN2A) gene, regulates the cell cycle by inhibiting the activity of cyclin-dependent kinases, specifically CDK4 and CDK6, which are involved in promoting cell cycle progression. In inhibiting these kinases, p16 helps prevent uncontrolled cell division and suppresses tumor formation (Li et al., 2012). Unlike p53, which is involved in a broader range of cellular responses, p16 is more specifically focused on cell cycle regulation. Its importance in cancer research is underscored by its frequent inactivation or loss in various cancers, leading to unrestrained cell proliferation. A highly defined P16 antibody is an essential tool in studying p16 as it enables researchers to assess expression levels, localization, and interactions, providing crucial insights into p16’s role in maintaining cell cycle control and its implications for cancer development and treatment. Our recombinant anti-p16 antibody is based on the clone 30859-S17 which has been cited for use in western blot, ELISA, and IHC applications. Additionally, we have engineered this clone from its original mouse IgG1 version into rabbit IgG to decrease cross-reactivity and background signal in human tissues.

KRAS 

KRAS mutations, specifically G12V, G12C, and G12D, are biomarkers associated with various cancers, particularly colorectal cancer and non-small cell lung cancer (Gao & Shen, 2022). These mutations result in the constitutive activation of the KRAS protein, leading to uncontrolled cell growth and tumor progression. Studying KRAS G12V, G12C, and G12D in cancer research using antibodies is crucial for understanding their expression patterns, localization, and activity levels. Like inactivated tumor suppressors, KRAS mutations represent an oncogenic driver, promoting tumorigenesis, and KRAS protein antibodies play a pivotal role in dissecting the aberrant signaling pathways associated with KRAS mutations, providing essential information for the development of targeted therapies. One such KRAS antibody is the anti-p21Ras clone DWP which has been cited for use in western blot, ELISA, and IHC and recognizes the KRAS mutant peptides G12V and G12C. Our recombinant version has been engineered from the original mouse IgG2b to such formats as mouse IgG2b Fc Silent™ with abrogated Fc binding for reduced non-specific staining. Other formats include human IgG1 with and without Fc binding functionality, and rabbit IgG.

TGF-beta 

Transforming Growth Factor-beta (TGF-β) is a multifunctional cytokine that plays a dual role in cancer, acting as both a tumor suppressor and a promoter, depending on the context (Seoane & Gomis, 2017). In its role as a tumor suppressor, TGF-β helps regulate cell growth, inhibit cell proliferation, and promote apoptosis. However, during later stages of cancer progression, TGF-β can paradoxically stimulate tumor growth and metastasis. Studying TGF-β in cancer research using antibodies is essential for elucidating its complex role in tumorigenesis. Antibodies enable researchers to investigate the levels, activity, and interactions of TGF-β, providing critical insights into its dynamic functions and potential as a therapeutic target in cancer treatment.

Other Recombinant Engineered Tumor Suppressor Antibodies 

Western blot using anti-E Cadherin antibody 19A11 (Ab03204).

  • Cadherin-1: Cadherins, particularly cadherin-1 (or E-cadherin) act as tumor suppressors by preserving cellular adhesion. Anti-cadherin antibodies are instrumental in researching tumor suppressors, allowing specific detection and quantification of cadherin levels, uncovering insights into mechanisms driving tumor progression.
  • Retinoblastoma protein: Retinoblastoma protein (Rb) is coded for by the RB1 gene, the first described human tumor suppressor gene (Berry et al., 2019), making Rb a pioneer protein for the study of tumor suppressors. Its importance in research has not dwindled, as Rb is central to cell cycle regulation and its downregulation and inactivation can lead to uncontrolled cell growth. We offer the recombinant engineered version of the anti-RBBP5 clone RAB-C379, cited for use in immunoprecipitation mass spectrometry and immunofluorescence assays.
  • SMARCA4: SMARCA4, also known as BRG1, is a tumor suppressor involved in chromatin remodeling. Mutations or loss of SMARCA4 function are observed in various cancers, notably small cell carcinoma of the ovary. Anti-SMARCA4 antibodies play a crucial role in studying this tumor suppressor, enabling specific detection and quantification, and providing insights into its contribution to cancer development. The anti-Brg1 clone 4E5 has published applications in immunoprecipitation, western blot, immunofluorescence, and immunohistochemistry. We recombinantly engineered anti-Brg1 [4E5] for more experimental options into various human, mouse, and rat IgG isotypes, including Fc Silent™ options on some formats for tailed effector function.
  • Catenin beta-1: Catenin beta-1 (encoded by CTNNB1), also known as β-catenin, is a key player in the Wnt signaling pathway and is integral to cell adhesion. Unique in the study of tumor suppressors, β-catenin functions both as a structural component in cell adhesion complexes and as a transcriptional co-activator in the nucleus. Dysregulation of β-catenin is often associated with various cancers, making anti-catenin beta-1 antibodies essential tools for specific detection and quantification. These antibodies contribute significantly to understanding the dual role of β-catenin and its involvement in cancer initiation and progression.

    IF staining of HeLa cells using anti-beta catenin 15B8 (Ab01655-23.0).

Why Go Recombinant? 

Absolute Antibody’s recombinant engineered antibodies, available in diverse formats and species, are essential tools for studying tumor suppressors. They enable specific detection and quantification of protein expression, including isoform-specific analysis. These antibodies facilitate functional studies, allowing manipulation of tumor suppressor activity, and can be utilized in high-throughput screening for drug discovery. Their species compatibility supports research across various organisms, contributing to the understanding of tumor suppressor biology.  

Additionally, the recombinant antibodies we offer are 100% biologically defined, opening further opportunities for our antibody engineers to customize these tools to your exact experimental needs. For more information on our custom services, we have resources available on formats we have made (including bi-, tri-, and multispecific formats), our recombinant production platform, and more. If your questions still aren’t answered, reach out! Our team is happy to help with any product questions or orders.

More Research Products 

As an Absolute Biotech brand, Absolute Antibody is proud to partner with our sister companies to provide an even more comprehensive menu of antibody-based research tools. Other reagents include: 

  • Human BRCA1 Antibody ELISA Kits from LSBio for the quantitative detection of Human BRCA1 in serum. Built for 96-well systems, this sandwich assay can detect BRCA1 down to 0.312 ng/ml. 
  • Monoclonal Mouse antiHuman SMAD4 Antibody from LSBio. These antibodies have been validated for IHC applications in frozen and paraffin-embedded tissues. 
  • Goat Anti-VHL Antibody from Everest Biotech. These polyclonal antibodies detect von Hippel-Lindau (VHL) tumor suppressor protein concentrations with high affinity in peptide ELISA, western blot, and IHC applications. 
  • Polyclonal Rabbit antiHuman APC Antibody from LSBio, reactive with human, mouse and rat. This polyclonal antibody has been validated for ELISA, IF, IHC, and western blot. 
  • Goat Anti-NUMB Antibody from Everest Biotech. This polyclonal antibody has been validated for peptide ELISA, IHC, IF, and flow cytometry. 

Tumor Suppressor Antibodies

Catalog IDTargetAntibody CloneFormats Available
Ab00142p53PAb421Mouse IgG2a
Rabbit IgG
Ab03148p5370237-85Rabbit IgG
Mouse IgG2a
Ab02776p53a.a. 16-25Mouse IgG1
Human IgG1
Human IgG1 Fc Silent
Rabbit IgG
Ab01907TP53BP1RAB-C425Human IgG1
Rabbit IgG
Mouse IgG1
Human Fab fragment
Mouse Fab fragment
Ab03149p1630859-S17Rabbit IgG
Mouse IgG1
Ab04234KRAS G12VG12V-34Mouse IgG1
Rabbit IgG
scFv fragment (His)
Ab03878KRAS G12C mutantAbAbKRAS01Human IgG1
Human IgG1 Fc Silent
Mouse IgG1
Rabbit IgG
Ab03877KRAS G12D mutantD113Rabbit IgG
Mouse IgG1
Human IgG1
Human IgG1 Fc Silent
Ab03876p21RasDWPMouse IgG2b Fc Silent
Human IgG1
Human IgG1 Fc Silent
Mouse IgG2b
Rabbit IgG
Ab03875p21RasKGH-R1Rabbit IgG
Mouse IgG2b
Human IgG1 Fc Silent
Human IgG1
Mouse IgG2b Fc Silent
Ab02466TGF-beta12H4Rabbit IgG
Human IgG1
Human IgG1 Fc Silent
Mouse IgG1
Ab03786TGF beta1SL15SHuman IgG1
scFv fragment (His)
Ab03951TGF beta1D11.16.8Rabbit IgG
Human IgG1
Human IgG1 Fc Silent
Mouse IgG1
Ab02465Latent TGF-beta12F10Rabbit IgG
Human IgG1
Human IgG1 Fc Silent
Mouse IgG1
Ab03702GDF15MBM-14Rabbit IgG
Mouse IgG2b Fc Silent
Mouse IgG2b
Human IgG1
Human IgG1 Fc Silent
scFv fragment (His)
Ab03701GDF15MBM-12Rabbit IgG
Mouse IgG2b Fc Silent
Mouse IgG2b
Human IgG1
Human IgG1 Fc Silent
scFv fragment (His)
Ab03700GDF15Clone 29Rabbit IgG
Mouse IgG2b Fc Silent
Mouse IgG2b
Human IgG1
Human IgG1 Fc Silent
Ab01853RBBP5RAB-C379Human IgG1
Rabbit IgG
Mouse IgG1
Human Fab fragment
Mouse Fab fragment
Ab00871p63BU5Mouse IgG2b Fc Silent
Rabbit IgG
Human IgG1
Mouse IgG2b
Ab03151p4014D5E8F12Rabbit IgG
Mouse IgG1
Ab03150p6360738-10D8Rabbit IgG
Mouse IgG2
Ab00923p21Y13-238Rat IgG2a
Rabbit IgG
Mouse IgG1
Mouse IgG2a
Mouse IgG2a Fc Silent
Human IgG1
Human IgG1 Fc Silent
Human IgG4-S228P
Ab03219p21Y13-259Rabbit IgG
Rat IgG1
Human IgG1
Human IgG1 Fc Silent
Ab02737GelsolinGsnVHH 13Human IgA-Fc fusion
Mouse IgG1-Fc fusion
Rabbit IgG-Fc fusion
Ab02738GelsolinGsnVHH 11 (Nb11)Human IgA-Fc fusion
Mouse IgG1-Fc fusion
Rabbit IgG-Fc fusion
Ab01654alpha catenin1G5Mouse IgG1
Rabbit IgG
Human IgG1
Human IgG1 Fc Silent
Human IgG4-S228P
Ab02695p120/catenin2B12Mouse IgG1
Human IgG1
Human IgG1 Fc Silent
Rabbit IgG
Ab01655beta catenin15B8Mouse IgG1
Rabbit IgG
Human IgG1
Human IgG1 Fc Silent
Human IgG4-S228P
Ab03193B-catenin3B1D11A8Rabbit IgG
Mouse IgG1
Human IgG1