Anti-FEN1 [SAIC-21C-4]

Description:

Recombinant monoclonal antibody to FEN1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the B-cell cDNA library SAIC-21C-4.

Clone Name:

SAIC-21C-4

Published Application(s):

immunoMRM

Published Species Reactivity:

Human

Products based on this clone:

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Ab00311-23.0 Anti-FEN1 [SAIC-21C-4]
Human
Rabbit IgG
Purified
In Stock

Ab00311-1.1 Anti-FEN1 [SAIC-21C-4]
Human
Mouse IgG1
Purified
In Stock

UniProt Accession Number of Target Protein: P39748

Alternative Name(s) of Target: Flap endonuclease; RAD2
Immunogen: The KLH-cojugated peptide "SIEEIVR", derived from the Flap endonuclease 1 involved in DNA replication and repair.

Specificity: Recognises human FEN1.

Application Notes: Original data characterizing this antibody may be found here .

Antibody first published in: Schoenherr et al. Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Mol Cell Proteomics PMID:25512614
Note on publication: Describes generation a panel of recombinant antibodies using synthetic peptides of human proteins as well as validation using ELISA and multiple reaction monitoring-mass spectrometry.

Flow cytometry analysis of HeLa cells using Ab00311-23.0. Click on image for more details.

Flow cytometry using the Anti-FEN1 antibody SAIC-21C-4 (Ab00311). Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of SAIC-21C-4 (Ab00311-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Western blot on human colon tissue lysate using Ab00311-23.0. Click on image for more details.

Western Blot using anti-FEN1 antibody SAIC-21C-4 (Ab00311). Human colon tissue lysates (35µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-21C-4 (Ab00311-23.0) at 1 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

IF staining of HeLa cells using Ab00311-23.0. Click on image for more details.

Immunofluorescence staining of HeLa cells with anti-FEN1 (Ab00311) SAIC-21C-4 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-21C-4 (Ab00311-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1500 dilution), showing nucleoli and nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00311-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.

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