Antibody Engineering Services

At Absolute Antibody we have developed a proprietary cloning system to enable rapid reformatting of antibodies into almost any format. Our antibody engineering services include chimerization, humanization, isotype switching and the manufacturing of recombinant antibody fragments and bispecific antibodies. For more information about any of these services, please follow the links, download our Guide to Antibody Engineering white paper, or get in touch – we’re happy to help.



The following species, isotypes and formats are currently available at Absolute Antibody with more coming soon. For more details about the unique formats we can custom engineer for clients, check out our Periodic Table of Antibodies.

Antibody format Human Mouse Rat Hamster Rabbit Goat Rhesus Cyno Sheep Dog Pig Ferret
Fc domains
Fc fusions

Other Species

If you require antibodies of a species not listed, please do not hesitate to ask. Currently we have antibody sequences available for a large number of animals ranging from pig, sheep, horse, dog, chicken, fish, platypus and a whole range of non-human primates. Moreover, our in-house experts are able to determine antibody sequences from most genomes (even if only partial assemblies are available). If you are looking for something very unusual, please contact us.


We offer an antibody chimerization service which enables you to create a chimeric antibody, consisting of the original variable domains from your antibody with the constant domains from a different species, for almost any mammalian species. In particular, we offer chimerization of human, mouse, rat, rabbit and hamster IgG with other species and isotypes available by request. For more information, please visit our dedicated page on chimeric antibodies or contact us.


We offer a royalty-free antibody humanization service. For more information, please see our dedicated page on antibody humanization or contact us.

Isotype switching

Our isotype switching service (also known as class switching) allows you to alter the isotype or subtype of any antibody. This can be used to alter the in vivo effector function and stability of an antibody. Equally, some antibody subtypes are prone to aggregation. Switching the isotype/subtype can help overcome this. Or perhaps you would like to convert your IgG to an IgM for increased avidity? We can offer all these things and more. For more information or to request a quote, contact us.

Antibody fragments

In certain situations antibody fragments offer an ideal alternative to full-length antibodies. Our proprietary recombinant expression and antibody engineering platform enables us to convert any antibody into a fragment, such as a single chain Fv (scFv), Fab or Fab2, followed by expression and purification at any scale. For more information, please visit our dedicated page on antibody fragments or contact us. For a general information guide on the types and uses of fragments, please see our Absolute Antibody resource page on antibody fragments.

Bispecific antibodies

Absolute Antibody offers a bispecific antibody service. We can reformat any pair of antibodies into an IgG-scFv format (i.e. an IgG with a scFv fused to the C-terminus of the IgG). This results in a bispecific bivalent antibody format capable of binding two antigens simultaneously. For more information, see our dedicated page on bispecific and trispecific antibodies or to request a quote please contact us.

Engineered Fc domains

Absolute Antibody can engineer the Fc domain of your antibody to mediate effector function or extend the plasma half-life. For example, we can abolish Fc domain binding to FcγR using our Fc Silent™ mutation or mAbsolve’s STR mutations, as well as extend or shorten the plasma half-life of your IgG antibody by introducing mutations in the Fc domain.

And more.

Below is a selection of the formats we have engineered for our customers. Contact us with your ideas – we’ll see what we can do! You can explore our interactive Periodic Table of Antibodies or download it as a PDF with the full legend here.