Antibody Engineering Services

At Absolute Antibody we have developed a proprietary cloning system to enable rapid reformatting of antibodies into almost any format. Our antibody engineering services include chimerization, humanization, isotype switching and the manufacturing of recombinant antibody fragments and bispecific antibodies. For more information about any of these services please follow the links or get in touch – we’re happy to help.

 

The following species, isotypes and formats are currently available at Absolute Antibody with more coming soon.

Antibody format Human Mouse Rat Armenian Hamster Rabbit Goat Rhesus Macaque Sheep
IgG
IgA
IgE
IgM
Fc domains
Fabs
Fab2
Fc-fusions
Bispecifics

Other Species

If you require antibodies of a species not listed please do not hesitate to ask. Currently we have antibody sequences available for a large number of animals ranging over pig, sheep, horse, dog, chicken, fish, platypus and a whole range on non-human primates. Moreover, our in-house experts are able to determine antibody sequences from most genomes (even if only partial assemblies are available). So if you are looking for something very unusual please contact us.

Chimerization

We offer an antibody chimerization service which enables you to create a chimeric antibody, consisting of the original variable domains from your antibody with the constant domains from a different species, for almost any mammalian species. In particular we offer chimerization of human, mouse, rat, rabbit and hamster IgG with other species and isotypes available on request. For more information or to request a quote, simply get in touch.

Humanization

We offer a royalty free antibody humanization service. For more information please see our dedicated page on antibody humanization or contact us.

Isotype switching

Our isotype switching service (also known as class switching) allows you to alter the isotype or subtype of any antibody. This can be used to alter the in vivo effector function and stability of an antibody. Equally, some antibody subtypes are prone to aggregation. Switching the isotype/subtype can help overcome this. Or perhaps you would like to convert your IgG to an IgM for increased avidity? We can offer all these things and more. For more information or to request a quote contact us.

Antibody fragments

In certain situations antibody fragments offer an ideal alternative to full length antibodies. Our proprietary recombinant expression and antibody engineering platform enables us to convert any antibody into a fragment, such as a single chain Fv (scFv), Fab or F(ab)’2, followed by expression and purification at any scale. For more information on our antibody fragment service or to request a quote please get in touch. For a general information guide on the types and uses of fragments please see our Absolute Antibody resource page on antibody fragments.

Bispecific antibodies

Absolute Antibody offers a bispecific antibody service. We can reformat any pair of antibodies into an IgG-scFv format (i.e. an IgG with a scFv fused to the C-terminus of the IgG). This results in a bispecific bivalent antibody format capable of binding two antigens simultaneously. For more information see our dedicated page on bispecific and trispecific antibodies or to request a quote please contact us.

And more.

Below is just a selection of the formats we have engineered for our customers. Contact us with your ideas – we’ll see what we can do.