United Kingdom (UK) Absolute Antibody’s Multi-Tag control protein (Pr00106) and Multi-Tag control protein 2 (Pr00565) are genetically engineered synthetic fusion proteins composed of commonly used epitope tags fused together using flexible linkers (Figure 1 and 2). These Multi-Tag synthetic fusion proteins can be used as a positive control in western/dot blots and ELISAs.
You can also check out our epitope tags page for our full range of recombinant and chimeric anti-epitope tag monoclonal antibodies. For further information on our Multi-Tag control proteins or any of our products, contact us and we will be happy to help.
Figure 1: A schematic representation of the Absolute Antibody’s Multi-Tag control protein (Pr00106), composed of 17 commonly used epitope tags shown in sequence from N- to C-terminal.
Figure 2: A schematic representation of the Absolute Antibody’s Multi-Tag control protein 2 (Pr00565), composed of 12 commonly used epitope tags shown in sequence from N- to C-terminal.
Table 1: List of tags found in Pr00106 and Pr00565.
| Multi-Tag control protein (Pr00106) | Multi-Tag control protein 2 (Pr00565) | ||
| Thioredoxin (Trx) tag
|
SDKIIHLTDDSFDTDVLKADGAILVDF
WAEWCGPCKMIAPILDEIADEYQGK LTVAKLNIDQNPGTAPKYGIRGIPTLLL FKNGEVAATKVGALSKGQLKEFLDAN LA |
MBP tag | MKIEEGKLVIWINGDKGYNGLAEVGKKFE
KDTGIKVTVEHPDKLEEKFPQVAATGDGP DIIFWAHDRFGGYAQSGLLAEITPDKAFQ DKLYPFTWDAVRYNGKLIAYPIAVEALSLI YNKDLLPNPPKTWEEIPALDKELKAKGKS ALMFNLQEPYFTWPLIAADGGYAFKYEN GKYDIKDVGVDNAGAKAGLTFLVDLIKNK HMNADTDYSIAEAAFNKGETAMTINGP WAWSNIDTSKVNYGVTVLPTFKGQPSKP FVGVLSAGINAASPNKELAKEFLENYLLTD EGLEAVNKDKPLGAVALKSYEEELAKDPRI AATMENAQKGEIMPNIPQMSAFWYAVR TAVINAASGRQTVDEALKDAQTNSSSNN NNNNNNNNLGIEGR |
| T7 tag | CMASMTGGQQMG | GCN4 tag | HLENEVARLKK |
| HSV tag | QPELAPEDPEDC | MAP-tag | GDGMVPPGIEDKIT |
| VSV-g tag | YTDIEMNRLGKG | Protein C tag | EDQVDPRLIDGK |
| Glu-Glu tag | CEEEEYMPME | Rap-tag | GDDMVNPGLEDRIEC |
| V5-tag | GKPIPNPLLGLDST | Rho-tag | TETSQVAPA |
| E-tag | GAPVPYPDPLEPR | Alpha-tag | SRLEEELRRRLTE |
| HA tag | YPYDVPDYAC | Rim-tag | PLHPRTAGASR |
| FLAG tag | DYKDDDDK | Softag | SLAELLNAGLGGS |
| GST tag
|
PILGYWKIKGLVQPTRLLLEYLEEKYEE
HLYERDEGDKWRNKKFELGLEFPNLP YYIDGDVKLTQSMAIIRYIADKHNML GGCPKERAEISMLEGAVLDIRYGVSRI AYSKDFETLKVDFLSKLPEMLKMFED RLCHKTYLNGDHVTHPDFMLYDALD VVLYMDPMCLDAFPKLVCFKKRIEAI PQIDKYLKSSKYIAWPLQGWQATFG GGDHPPKSDLV |
Strep tag | WSHPQFEK |
| Myc tag | EQKLISEEDLC | His tag | HHHHHH |
| S-tag | KETAAAKFERQHMDS | Pep tag | AVERYLKDQQLLGIW |
| KT3 tag | KPPTPPPEPET | ||
| E2-tag | GVSSTSSDFRDR | ||
| AU1 tag | DTYRYI | ||
| AU5 tag | TDFYLK | ||
| His tag | HHHHHH | ||
| Catalog Number | Product Name | Intro Price | Availability | Product Page |
|---|---|---|---|---|
| Pr00106 | Multi-Tag Control Protein | 0.2 mg for £225 / €260 / $285 | In stock, contact us | View Datasheet |
| Pr00565 | Multi-Tag Control Protein 2 | 0.2 mg for £225 / €260 / $285 | In stock, contact us | View Datasheet |