CHXpress™ CHO Antibody Production

Absolute Antibody recently expanded its recombinant antibody expression services with the addition of the CHXpress™ transient CHO expression service. The addition builds upon our established HEXpress™ platform, enabling us to offer serum-free mammalian transient expression in both HEK and CHO cells to better serve clients through their full antibody development.

We still recommend our cost-effective HEK expression platform for early-stage antibody development, as well as all reagent and diagnostics antibodies, but transient CHO production at milligram-to-gram scales is particularly useful for bridging the gap to stable CHO production, or for tackling challenging constructs. It enables therapeutic antibody developers to quickly manufacture and screen a group of antibodies before taking the time to generate stable cell lines for their most promising candidates. While stable cell line generation typically takes six months to a year, our transient CHO platform can produce high quality recombinant antibodies in engineered formats within a month.

We can start expression from your electronic antibody sequence, hybridoma cell line or purified antibody protein. We offer milligram-to-gram scale expression of antibodies in any species, isotype or format, with all production occurring in our ISO 9001:2015-certified manufacturing facility. Our Gold and Silver expression services provide highly purified IgG antibodies suitable for in vitro and in vivo needs, while our antibody engineering expertise enables the manufacture of unique formats such as multispecifics and fragments.

Read about our different CHO expression services below. To learn more, download our application note about the development and characterization of our transient CHO expression platform. The data demonstrate that the platform produces high quality, fully glycosylated human IgG antibodies with batch-to-batch reproducibility, as well as antibodies in engineered formats with reliably robust production yields and expected binding activity.

Contact us or fill out the form below to start a discussion about your project.

CHXpress™ Silver Antibody Expression Service

The CHXpress™ Silver service offers high-quality recombinant antibody expression at affordable prices, with our academic customers receiving a further 10% discount. The service covers gene synthesis to highly purified antibody in as little as 6 weeks.

Customer provides Scale and format Standard service details Timeline Deliverables
Electronic sequence or hybridoma 1 mg to 2 grams
Any IgG species or subtype
  • Gene synthesis
  • Cloning
  • Affinity purification
  • Buffer exchange (PBS)
  • Sterile filtration
  • Purity >96% (SDS-PAGE)
  • Endotoxin < 1EU/mg
6-8 weeks
  • Purified antibody
  • QC data

CHXpress™ Gold Antibody Expression Service

The CHXpress™ Gold service has been developed for those in pre-clinical development of therapeutic antibodies. This service provides the same speed as the standard service but with stricter control on monomer purity and endotoxin levels.

Customer provides Scale and format Premium service details Timeline Deliverables
Electronic sequence or hybridoma 1 mg to 2 grams

Any IgG species or subtype

  • Cloning
  • Affinity purification
  • Size exclusion chromatography
  • Sterile filtration
  • Monomer purity >98% (HPLC)
  • Endotoxin <0.5 EU/mg
6-8 weeks
  • Purified antibody
  • QC data

Engineered Antibody Expression Service

Absolute Antibody specializes in antibody engineering and the recombinant expression of antibodies in any format. Our Periodic Table of Antibodies illustrates the more than 180 different antibody formats we’ve produced to date, with our real-world expression experience informing the manufacturability score of each antibody.

For engineered antibodies, we begin with a milligram-scale pilot production run to determine expression levels and purification strategy before moving onto larger-scale production. We use a variety of antibody purification strategies to enable manufacturing success across such a wide range of formats, including a proprietary lectin-based purification system for our IgA, IgM and IgE antibodies. Each engineered antibody is different, and our pilot run will inform further and larger-scale production to ensure we deliver antibodies of the highest possible quality.

Contact Us About Your Antibody Expression Project

Fill out the form below to start a conversation about your antibody expression needs. A sales representative will be in touch shortly to discuss details, review pricing and answer any questions.
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